[Histonet] using HEIR and PIER together in one protocol, recommendations?

[Geraci-Erck, Maria]

Fellow Histonetters,

I am using CD90.1 to label rat tissue paraffin sections with variable
results.  I have tried all the conventional methods and would like to
try using enzyme digestion with Heat Induced AR.  Does anyone have any
recommendations?  Do you routinely use one retrieval before the other?
Are there any references out there to refer to?  I did a quick search
and did not find any references for this methodology.  I look forward to
hearing from the Histonet.

Maria Geraci-Erck
Schering-Plough Research Institute
Special Techniques Laboratory

maria.geraci-erck <@t> spcorp.com




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