[Histonet] OX-42

[Anand Vasudevan]

Hi,
I am doing antibody staining with mouse anti-rat Ox-42/CD11b/c (from
millipore, CBL1512). I have tried staining at room temperature and at 4C but
the either background staining is high (with use of detergent- 0.3% triton-x
100) or the staining is very light (with no detergent) at even 1:1000
dilution of the primary antibody. I use horse anti-mouse IgG secondary
antibody and I am developing the stain with DAB.

Is there anyway to increase the staining intensity other than increasing the
antibody concentration?

By the way I can find primary antibody of OX-42 only raised in mouse and not
any other species (and all the other papers I have seen). Does anyone know
the reason why?

Thanks,
Anand Vasudevan

School of Biological Sciences
NTU
Singapore