[Histonet] Embedding and Sectioning of tissue containing Gels.

[Melanie Black]

Hi All

We are looking at rat issue which has had a gel subcutaneously  
injected into it. We have routinely processed it and embedded it into  
was. The gel appears to survive processing and embedding, but then  
seems to be pulled out or distorted during sectioning. We would like  
to visualize it in tact to look at cells within the gel.

I have alternately embedded it in JB 4 resin (which is water soluble  
and does not require dehydration. This method is so far the best,  
however there are still some challenges.
i) There are sometimes bubbles in the resin that occur during  
polymerization.
ii) Some gels, depending on formulations don't take up the resin  
entirely, and therefore are still too soft to section.

We use a glass knife to section this resin. Staining is good, and  
immunofluorescence in the form of ED-1 and Actin are successful.

Does anybody have any experience with this type of research related  
problem?

Many Thanks
Melanie Black

Melanie Black
082 469 3352

Cardiovascular Research Unit
3rd Floor; Chris Barnard Building
Medical School;
Observatory. 7925.
University of Cape Town.
South Africa.