[Histonet] Re: Brain frozen sections

[Anne van Binsbergen]

HA!
excellent - I ran a neuropath lab for 8 years and we ALWAYS used smears - I
used to make the smears and stain the slide - by the time the Path go to the
lab all he had to do was read them!!
...but here it is different - they insist on frozens and my techs know that
most of the time there will be frezzing artefact
I will pass the message to them and then I will run and hide!! LOL ;D
thanks
greetings from the desert
Anne

2009/10/7 Tim Wheelock <twheelock <@t> mclean.harvard.edu>

> Hi:
>
> There is one thing you might try to make frozen brain sections more than
> acceptable.
> You can freeze the tissue in liquid nitrogen vapor (LNV) (not directly into
> liquid nitrogen), let the tissue equilibrate to cryostat temperature, and
> then cut the sections.
> The histology is almost as good as a paraffin section.
>
> This is assuming that the technique fits the time frame within which you
> must arrive at a diagnosis.
> Also we ourselves do not use frozen sections to screen brains
> diagnostically. We use paraffin sections from the formalin fixed half of the
> brain.
> We send the LNV frozen blocks to investigators who cut sections from the
> frozen blocks for their research.
>
> If you would like the details, you can contact one of the following
> individuals who do this routinely at our brain bank, to see if  this
> protocol is appropriate for your needs.
>
>
>
> George Tejada
> 617-855-2646
> gtejada <@t> mclean.org
>
> Louis Fernandes
> 617-855-2636
> lfernandes <@t> mclean.harvard.edu
>
>
> Good luck.
> I hope this information helps.
>
>
>
> Tim Wheelock
> Assistant Director, Neuropathology
> Instructor In Neuroanatomy
> Harvard Brain Tissue Resource Center
> 203 Mailman Research Center
> McLean Hospital
> Belmont MA 02478
> Phone: 617-855-3592
> Fax: 617-855-3199
>
>
>
> Robert Richmond wrote:
>
>> You guys tell your pathologists to listen to Dr. Hessler - he taught
>> me how to do these preparations, and the basics of interpreting them,
>> when I did a locum tenens for him at the Medical College of Georgia
>> (in Augusta) 6 years ago. I did a lot of them when I got a full time
>> job at a place that did a lot of neurosurgical pathology, a year
>> later.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Knoxville TN
>> *********************
>> There is nothing you can do to make brain frozen sections acceptable.
>> Your Pathologists need to learn how to read smears, or just accept
>> being wrong 50% of the time. An educated guess based on the imaging is
>> more accurate than frozen sections on intra-axial primary brain
>> tumors.
>>
>> Richard B Hessler, MD
>> Chief of Pathology
>> Erlanger Medical Center
>> Chattanooga, TN
>>
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>>
>>
>
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE