[Histonet] frozen myocardium sections

[Alan Bright]

Perhaps try freezing @ a higher temperature e.g C02.
Regards

Alan Bright
Bright Instrument Co.
Cambridgeshire
UK
Sent from my BlackBerry® wireless device

-----Original Message-----
From: "Jean-Martin Lapointe" <jm.lapointe <@t> accellab.com>
Date: Tue, 6 Oct 2009 10:08:03 
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] frozen myocardium sections

Hi all,

for a study we are freezing myocardium sections in OCT immersed directly
in liquid nitrogen, without isopentane, because apparently isopentane
quenches the fluorescence of the cells we need to detect in the tissue.
Unfortunately the blocks tend to crack after freezing. Does anyone have
a suggestion to avoid cracking ?

thanks

 

__________________________________

Jean-Martin Lapointe

AccelLAB Inc

jm.lapointe <@t> accellab.com <mailto:jm.lapointe <@t> accellab.com> 

 

 

 

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