[Histonet] Brain Frozen sections
Richard Hessler, M.D.
rhessler <@t> pathgroup.com
Mon Oct 5 12:21:00 CDT 2009
There is nothing you can do to make brain frozen sections acceptable. Your Pathologists need to learn how to read smears, or just accept being wrong 50% of the time. An educated guess based on the imaging is more accurate than frozen sections on intra-axial primary brain tumors.
Richard B Hessler, MD
Chief of Pathology
Erlanger Medical Center
Chattanooga, TN
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Subject: Histonet Digest, Vol 71, Issue 5
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Today's Topics:
1. Desperately need frozen kidney (Scott Gill)
2. Brain Frozen sections (Anne van Binsbergen)
3. RE: Histobath Frozen Sections (Galbraith, Joe)
4. time in paraffin and fried bloody specimen (Nancy Schmitt)
5. Leica Bond/Novocastra Reagents (Paula Lucas)
6. RE: Leica Bond/Novocastra Reagents (Josie Britton)
7. RE: Leica Bond/Novocastra Reagents (Joanne Mauger)
8. Re: Leica Bond/Novocastra Reagents (Greg Dobbin)
9. RE: Leica Bond/Novocastra Reagents (Weems, Joyce)
10. Re: time in paraffin and fried bloody specimen (Rene J Buesa)
11. Re: time in paraffin and fried bloody specimen (DKBoyd <@t> chs.net)
----------------------------------------------------------------------
Message: 1
Date: Sun, 4 Oct 2009 20:39:00 -0700 (PDT)
From: Scott Gill <histotemp <@t> yahoo.com>
Subject: [Histonet] Desperately need frozen kidney
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <573555.19370.qm <@t> web58303.mail.re3.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello,
? My research project is in desperate need of a couple fresh frozen pieces of kidney positive for C3 and/or C4.? (lupus?)? I will gladly pay any costs to get the tissue sent.? (boxes, dry ice, postage, etc.)
? Thanks in advance for your time
Scott
histotemp <@t> yahoo.com.?
------------------------------
Message: 2
Date: Mon, 5 Oct 2009 08:38:06 +0400
From: Anne van Binsbergen <annigyg <@t> gmail.com>
Subject: [Histonet] Brain Frozen sections
To: "histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<f8332fbe0910042138r2ecd55b7ve63e503ec6afcdcb <@t> mail.gmail.com>
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Hi Histonetters
we are having INTERMITTENT problems with brain frozens
all conditions are the same ie. same tech, same cryostat, same freezing
technique - sometimes there is freezing artefact in the sections and
sometimes the sections look like they were cut from an FFPE block!!
unfortunately not all our pathologists are confident to make an IOC
diagnosis on a smears so we are forced to resort to freezing.
my tech is very experieince and says she can almost predict when looking at
the tissue, that there will be artefact on the sections.
pathologists are insisting on the FZ but complaining bitterly at the
freezing artefact.
I suspect the surgeon may be 'flooding' the tissue with saline but unless we
breathe down their necks we cannot possibly know what goes on in the OR!
All other frozens are fine - its just the very small neuro ones we are
having a problem with and it is driving us to distraction - well, actually
the Pathologists are doing that!!
we are using cryospray, gently sprayed onto the tissue, which is
covered with a small drop of fresh OCT, on top of an already frozen button
of OCT which is kept in the cryostat
there is no problem with other frozen sections, on other tissues, prepared
in exactly the same way
any and all observations/recommendations/suggestions will be greatfully
accepted
TIA
--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
------------------------------
Message: 3
Date: Mon, 5 Oct 2009 09:16:41 -0500
From: "Galbraith, Joe" <joseph-galbraith <@t> uiowa.edu>
Subject: RE: [Histonet] Histobath Frozen Sections
To: <mwhite <@t> mcleodhealth.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AEECB16E79B57342806128D46AF1687905C91450 <@t> HC-MAIL10.healthcare.uiowa.edu>
Content-Type: text/plain; charset="us-ascii"
Melanie:
We are acquiring a SnapFrost 2 unit from Alphelys. They offer two
models - one at -80 and one at -100.
Joe
joseph-galbraith <@t> uiowa.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
mwhite <@t> mcleodhealth.org
Sent: Wednesday, September 23, 2009 2:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Histobath Frozen Sections
Histonetters:
In our sister lab, the Histobath freezing unit has died. We need to know
if
there is another comparable product available, since the Histobath is no
longer manufactured. Our pathologists don't care for the peltier option.
We researched the archives and found that this has been discussed on the
Histonet in the past, but didn't see many responses. There is a similar
(but larger, if I understand correctly) unit available from a vendor in
the UK.
Can anyone contribute suggestions or comments?
Thanks in advance.
P.S. Are there any other Samurai Pathologists available? We need one.
Melanie S. White, MT(ASCP)
Laboratory Supervisor, Systems/Anatomic Pathology
McLeod Regional Medical Center
(843) 777-2072
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Message: 4
Date: Mon, 5 Oct 2009 10:06:31 -0500
From: Nancy Schmitt <nancy_schmitt <@t> pa-ucl.com>
Subject: [Histonet] time in paraffin and fried bloody specimen
To: "Histonet (histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<737BD0BF52F0744B96B74B61756AC0644145E938BF <@t> hestia.ad.pa-ucl.com>
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Good Morning Histonetters-
First question: Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening". Can anyone define "exposure to prolonged heat"? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this.
Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue.
Thanks for your help!
Nancy
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Message: 5
Date: Mon, 05 Oct 2009 08:20:06 -0700 (PDT)
From: Paula Lucas <plucas <@t> biopath.org>
Subject: [Histonet] Leica Bond/Novocastra Reagents
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <20091005152006.93D578882 <@t> arkroyal.cnchost.com>
Content-Type: text/plain; charset="utf-8"
Hello,
We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away?
Thank you,
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
------------------------------
Message: 6
Date: Mon, 5 Oct 2009 11:29:12 -0400
From: "Josie Britton" <JCBRITTON <@t> Cheshire-Med.COM>
Subject: RE: [Histonet] Leica Bond/Novocastra Reagents
To: "Paula Lucas" <plucas <@t> biopath.org>, <histonet <@t> pathology.swmed.edu>
Message-ID:
<F644CD64B2313F43BB3D2496B454CDAC038C2216 <@t> CMC-EX01.cheshire-med.com>
Content-Type: text/plain; charset="us-ascii"
We get almost all their antibodies and ancillaries and have never had a
problem! They are a great company and we love the Bond Max.
Josie Britton HT
Cheshire Medical Center
Keene, NH 03431
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Monday, October 05, 2009 11:20 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Leica Bond/Novocastra Reagents
Hello,
We are considering the Leica Bond and their reagent rental or
acquisition option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents
(novocastra), are there any problems with back orders on antibodies,
detection system and other reagents needed to run the Bond? Or, do you
usually get the items right away?
Thank you,
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
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------------------------------
Message: 7
Date: Mon, 05 Oct 2009 11:41:44 -0400
From: "Joanne Mauger" <MAUGER <@t> email.chop.edu>
Subject: RE: [Histonet] Leica Bond/Novocastra Reagents
To: <plucas <@t> biopath.org>,<JCBRITTON <@t> Cheshire-Med.COM>,
<histonet <@t> pathology.swmed.edu>
Message-ID: <4AC9DB780200003100014828 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII
We have never had a backorder problem- have 2 Bondmax's over 2 years. I love them.
Jo Mauger
>>> "Josie Britton" <JCBRITTON <@t> Cheshire-Med.COM> 10/05/09 11:29 AM >>>
We get almost all their antibodies and ancillaries and have never had a
problem! They are a great company and we love the Bond Max.
Josie Britton HT
Cheshire Medical Center
Keene, NH 03431
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Monday, October 05, 2009 11:20 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Leica Bond/Novocastra Reagents
Hello,
We are considering the Leica Bond and their reagent rental or
acquisition option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents
(novocastra), are there any problems with back orders on antibodies,
detection system and other reagents needed to run the Bond? Or, do you
usually get the items right away?
Thank you,
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
_______________________________________________
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------------------------------
Message: 8
Date: Mon, 05 Oct 2009 12:48:45 -0300
From: "Greg Dobbin" <gvdobbin <@t> ihis.org>
Subject: Re: [Histonet] Leica Bond/Novocastra Reagents
To: <plucas <@t> biopath.org>,<histonet <@t> pathology.swmed.edu>
Message-ID: <sac9eb35.094 <@t> ihis.org>
Content-Type: text/plain; charset=US-ASCII
Almost always receive on time. I can think of only one instance in a
year and a half where anything was on back order (it was an antibody).
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
"I find that the harder I work, the
more luck I seem to have."
- Thomas Jefferson
>>> Paula Lucas <plucas <@t> biopath.org> 10/5/2009 12:20 PM >>>
Hello,
We are considering the Leica Bond and their reagent rental or
acquisition option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents
(novocastra), are there any problems with back orders on antibodies,
detection system and other reagents needed to run the Bond? Or, do you
usually get the items right away?
Thank you,
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-------------------------
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Message: 9
Date: Mon, 5 Oct 2009 11:49:35 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Leica Bond/Novocastra Reagents
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<B36E22177C738742AE407DBA366FFD4A0816EE <@t> ITSSSXM02V1.one.ads.che.org>
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We've had no problems with either Bond or Novacastra orders.
Joyce
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Monday, October 05, 2009 11:20
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Leica Bond/Novocastra Reagents
Hello,
We are considering the Leica Bond and their reagent rental or
acquisition option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents
(novocastra), are there any problems with back orders on antibodies,
detection system and other reagents needed to run the Bond? Or, do you
usually get the items right away?
Thank you,
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
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Message: 10
Date: Mon, 5 Oct 2009 09:04:27 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] time in paraffin and fried bloody specimen
To: "Histonet \(histonet <@t> lists.utsouthwestern.edu\)"
<histonet <@t> lists.utsouthwestern.edu>, Nancy Schmitt
<nancy_schmitt <@t> pa-ucl.com>
Message-ID: <345606.14124.qm <@t> web65711.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
After you have developed a processing protocol and?obtained good infiltration after a certain time (hours) in paraffin, any and all the time above that period of adequate infiltration = exposure to prolonged heat.
Some histotechs even don't fill the holding chamber in the embedding center, a practice I do not think is adequate.
To your second question, just place them in NBF and when fixed filter and wrap them yourself while cassetting, do not wrap them before being fixed.
Ren? J.
--- On Mon, 10/5/09, Nancy Schmitt <nancy_schmitt <@t> pa-ucl.com> wrote:
From: Nancy Schmitt <nancy_schmitt <@t> pa-ucl.com>
Subject: [Histonet] time in paraffin and fried bloody specimen
To: "Histonet (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, October 5, 2009, 11:06 AM
Good Morning Histonetters-
First question:? Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening".? Can anyone define "exposure to prolonged heat"?? Is that an hour? Three hours?? Sitting in the paraffin waiting to be drained.? I would appreciate some insight on this.
Second question:? Endom, POC tissue, even some sinus contents arrive wrapped in lens paper.? These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper.? Is there something different we or the PA can be doing differently or just the nature of the tissue.
Thanks for your help!
Nancy
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Message: 11
Date: Mon, 5 Oct 2009 12:11:54 -0400
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] time in paraffin and fried bloody specimen
To: Nancy Schmitt <nancy_schmitt <@t> pa-ucl.com>
Cc: "Histonet \(histonet <@t> lists.utsouthwestern.edu\)"
<histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
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<OF406F22CC.C7789137-ON85257646.0056C555-85257646.0058F1AA <@t> chs.net>
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Nancy,
Tissue should be processed @ between 60-62 degrees centigrade. We have
three paraffin baths. The 1st bath is set for 45 mins, the 2cd and 3rd
are for 1 hour each. This is for large specimens. Small specimens are
for 30 mins. the first two baths and 45 mins for the last. It is very
true that too much time in paraffin causes hard tissue. Remember the
whole time the tissue is setting in paraffin it is being exposed to heat.
Your second question: Have the specimen transferred from the lens paper
it arrived in and put on a new piece which has been moistened with
formalin. Sometimes in surgery the lens paper is wet with saline.
If it is a scant amount process with your Endoscopic biopsies. Too long in
your alcohols will over dehydrate the specimen.
Hope this helps.
Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical
Center I
200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F:
804-765-5582 I dkboyd <@t> chs.net
Nancy Schmitt <nancy_schmitt <@t> pa-ucl.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/05/2009 11:07 AM
To
"Histonet (histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] time in paraffin and fried bloody specimen
Good Morning Histonetters-
First question: Textbook says "tissue should remain in paraffin the
shortest time necessary for good infiltration because exposure to
prolonged heat causes shrinkage and hardening". Can anyone define
"exposure to prolonged heat"? Is that an hour? Three hours? Sitting in
the paraffin waiting to be drained. I would appreciate some insight on
this.
Second question: Endom, POC tissue, even some sinus contents arrive
wrapped in lens paper. These bloody specimens are fried (for lack of a
better word) and almost impossible to separate from the lens paper. Is
there something different we or the PA can be doing differently or just
the nature of the tissue.
Thanks for your help!
Nancy
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is for the use of only the intended recipient(s) even if addressed
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that you have received it in error and then delete it along with any
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