[Histonet] dehydration

[Rene J Buesa]

It seems that you are missing the "clearing" step or antemedium in the sequence you describe but, the tissue should not stay too long in neither alcohol (unless it is 2-propanol) or in the antemedium (unless it is mineral oil) in which cases the time in them is not an issue.
René J.

--- On Thu, 3/19/09, Dianne Holmes <dholmes <@t> anatomy.umsmed.edu> wrote:

From: Dianne Holmes <dholmes <@t> anatomy.umsmed.edu>
Subject: [Histonet] dehydration
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, March 19, 2009, 2:54 PM

In an emergency situation (like not enough hrs in a day) - how long can tissue
remain in the final ETOH before going into the paraffin embedding stage?  I do
all my work manually - no automation here!!


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