[Histonet] RE: regarding muscle bx containers containers

Thu Jul 30 08:45:45 CDT 2009

Received confirmation on correct number from Fisher on muscle storage containers: However.... here is reply from staff...

"Carol
I called Fisher this morning and had them check; it is the correct number and is on the website. They may however have to order directly from Fisher.
Terry" (McLaughlin,Histo...Core Lab)

Hope this helps...the containers make a big difference in dehydration/or not.....We use only this type of container for storing muscle bx's for histochem(along with the frozen water droplet inside).  If it is surface freeze thaw artifact...try to cut a little past with a face cut, or two, in taking your first cut... and remember not to cut anything that is down in the freezing medium.  It will always have artifact. 

If the sample is "cracking"...you may be keeping it too long in the isopenatane at the initial snap-freeze...Muscle bx's are a creature of their own. Let me know if this helps! Lots of luck!!!!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 9:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68, Issue 39

Send Histonet mailing list submissions to
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Today's Topics:

   1. RE: SLIDE PRINTER (Laurie Colbert)
   2. 1. Artifact in Muscle bx's frozen for extended time	(Sharon
      Allen) (Walter Benton)
   3. urine cytology with red blood cells (Hayes, Tina J.)
   4. Re: Mouse Eyes (Paula Pierce)
   5. RE: Artifact in long stored muscle bx (Barone, Carol )
   6. RE: SLIDE PRINTER (Jimmy Markum)
   7. RE:Muscle Artifact (Barone, Carol )
   8. RE: Muscle Artifact (Barone, Carol )
   9. Problems with IHC (Mike Valerio)
  10. RE: Artifact in  Muscle bx's frozen for extended time
      (Ingles Claire )
  11. Glycophorin A antibody (Justin Peters)
  12. RE: flash frozen tissue (Ingles Claire )
  13. RE: RE: Muscle Artifact (Walters, Katherine S)
  14. slide and cassette labelers (Patsy Ruegg)
  15. Eric M. Halpern/CompHealth is out of the office.
      (eric.halpern <@t> comphealth.com)
  16. Re: SLIDE PRINTER (Andrea Grantham)
  17. Antigen retrieval paraffin skin samples (Nicholas David Evans)
  18. Re: urine cytology with red blood cells (njoydobro <@t> aol.com)
  19. RE: Artifact in  Muscle bx's frozen for extended time
      (Tony Henwood)

----------------------------------------------------------------------

Message: 1
Date: Wed, 29 Jul 2009 10:04:46 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: RE: [Histonet] SLIDE PRINTER
To: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<57BE698966D5C54EAE8612E8941D76830628C3C1 <@t> EXCHANGE3.huntingtonhospital.com>

Content-Type: text/plain;	charset="us-ascii"

We have two units that we bought from Accuplace when it was called the
PSLIM.  We had a fair amount of problems with them and I was ready to
return them, but Accuplace sent someone out to service them, and they
are working really well.  I've heard that when Fisher took over the
slide printer, they "revamped" it, so that it would have less problems.
We have the Thermo Fisher Printmate cassette labeler, and we have a 2D
barcode print on the cassette.  The PSLIM (Slide Mate) reads the barcode
and prints out the number of slides that we tell it to.  We have it
programmed very simply right now, but it is flexible and you are able to
have it print various ways.  I love it!

Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Janice
Mitchell
Sent: Wednesday, July 29, 2009 8:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] SLIDE PRINTER

Anyone out there in Histoland have any experience with Thermo slide
mate?  We are looking into purchasing a few.   Thanks for any input,
Janice

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 2
Date: Wed, 29 Jul 2009 13:27:12 -0400
From: "Walter Benton" <WBENTON <@t> umm.edu>
Subject: [Histonet] 1. Artifact in Muscle bx's frozen for extended
	time	(Sharon Allen)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A704E30.D886.00F4.3 <@t> umm.edu>
Content-Type: text/plain; charset=US-ASCII

Sharon,

We process quite a few samples as well and I did them at Hopkins with a similar time frame for the old tissue. We did not experience the problems you are referencing when we pulled the cases out from storage. What is the exact type of artifact that you are seeing? Is it freeze/thawing artifact or is it the tiny holes that come from water crystals? I could send you our freezing technique if you like. I would also suggest using cryo vials that have rubber gaskets which are designed for use in  those extreme temperatures. 

Walter Benton
Histology Supervisor
University of Maryland
Anatomic Pathology
22 S. Greene St 
Room NBW65
Baltimore MD 21201
(Direct) 410-328-0930
(Lab) 410-328-5524
(Fax) 410-328-5508

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------------------------------

Message: 3
Date: Wed, 29 Jul 2009 13:54:46 -0400
From: "Hayes, Tina J." <Tina.Hayes <@t> va.gov>
Subject: [Histonet] urine cytology with red blood cells
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<01A34750423B874399B8AF6674D90A270420A87D <@t> VHAV01MSGA1.v01.med.va.gov>
Content-Type: text/plain;	charset="us-ascii"

Our pathologist would like us to set up a procedure for cytologies
(urines, in particular) that will NOT lyse red blood cells.  Does
anybody know of a transport/preservation medium for urine cytologies
that does not lyse RBC's?  And is anybody using it with ThinPrep?  We
are currently using ThinPrep with Cytolyt, however the Cytolyt solution
is designed to lyse the RBC's.

------------------------------

Message: 4
Date: Wed, 29 Jul 2009 11:49:54 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: Re: [Histonet] Mouse Eyes
To: "Jo-Ann Bader, Ms." <jo-ann.bader <@t> mcgill.ca>,	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <213548.97351.qm <@t> web1113.biz.mail.sk1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Do they also want undetached retina? If so, do not use NBF to fix.

Paula Pierce, BA, AAS, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway Ave.
Moore, OK 73160
405-759-3953
Specializing in whole eye histology. All species.

________________________________
From: "Jo-Ann Bader, Ms." <jo-ann.bader <@t> mcgill.ca>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, July 29, 2009 10:38:05 AM
Subject: [Histonet] Mouse Eyes

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Wed, 29 Jul 2009 15:09:19 -0400
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] RE: Artifact in long stored muscle bx
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<37E4BAC017F57141AF64FAA5AEB04CE8033A74B2 <@t> wlmmsx01.nemours.org>
Content-Type: text/plain; charset=iso-8859-1

Have also been doing them since 1982....know what you mean about the artifact. 

We store in Bell-Art containers (availavle thru Fisher)..We have found no others to have the ability to storewithout cracking for so long.

We do place about 100 ul of water in the storage container...and place it in the LN for a quick freeze before we place any sample in the container, to hydrate over long period of time. 

Some other hint's...we use 7-8% trangacanth gum for mounting samples....and place it only at the base of the mounted tissue.  Once tissue is down into the medium....you will always have artifact.

 Note also: if you are snap freezing in isopentane, be sure to allow the isopentane to completely evaporate from the same (in a cryo)until it appears dry, before placing in the container. Isopentane left on the samples causes the same sort of artifact. This are my best suggestions....I wold love to hear any others though....always nice to learn more. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68, Issue 38

Send Histonet mailing list submissions to
	histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
	histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."

Today's Topics:

   1. Artifact in  Muscle bx's frozen for extended time (Sharon Allen)
   2. flash frozen tissue (Kalleberg, Kristopher)
   3. Mouse Eyes (Jo-Ann Bader, Ms.)
   4. SLIDE PRINTER (Janice Mitchell)
   5. Re: Mouse Eyes (Rene J Buesa)

----------------------------------------------------------------------

Message: 1
Date: Wed, 29 Jul 2009 09:27:24 -0500
From: "Sharon Allen" <SAllen <@t> exchange.hsc.mb.ca>
Subject: [Histonet] Artifact in  Muscle bx's frozen for extended time
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<BB6ADCD4B7ABB045A09A7634AC15CC610BEC8077 <@t> hscxmsmx0010.ad.wrha.mb.ca>
Content-Type: text/plain; charset="iso-8859-1"

Hi,
I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70°C freezer.  We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum,  I am assuming from drying out from months/years of freezing.
We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them.  We store them in a small plastic tube with a screw top.
I would like to know if anyone has further methods to eliminate this artefact.
Possibly sealing with OCT?  Placing water in the bottom of the tube?   Wrapping the muscle bx in Parafilm, tin foil etc?
What containers do you use to store them in if you do not get this artefact?
Suggestions for long term storage would be very much appreciated.
Thanks
Sharon Allen
Senior Neuropathology Technologist
HSC
WPG, MB, CA
This email and/or any documents in this transmission is intended for the
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Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original.

------------------------------

Message: 2
Date: Wed, 29 Jul 2009 10:31:39 -0400
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] flash frozen tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<0E6BC087F70F9C47ACFF2C203D6E329C067F7868 <@t> NTRSEVS30002.s3.ms.unilever.com>

Content-Type: text/plain;	charset="us-ascii"

Hello All,

I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen.  My big concern is what is the proper way to fix all of these SKIN samples to use for IHC.  Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best.  Any and and all help will be greatly appreciated.  Thank you.

Kris Kalleberg    

------------------------------

Message: 3
Date: Wed, 29 Jul 2009 11:38:05 -0400
From: "Jo-Ann Bader, Ms." <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<76D119EF12C904418800ED67CCB2062905E2AB9071 <@t> EXMBXVS1.campus.mcgill.ca>
Content-Type: text/plain; charset="us-ascii"

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6

------------------------------

Message: 4
Date: Wed, 29 Jul 2009 11:42:27 -0400
From: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>
Subject: [Histonet] SLIDE PRINTER
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A7035A3020000000052CB35 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII

Anyone out there in Histoland have any experience with Thermo slide mate?  We are looking into purchasing a few.   Thanks for any input, Janice

------------------------------

Message: 5
Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>,	" Ms.Jo-Ann Bader"
	<jo-ann.bader <@t> mcgill.ca>
Message-ID: <950812.3007.qm <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration.
René J.

--- On Wed, 7/29/09, Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca> wrote:

From: Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, July 29, 2009, 11:38 AM

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 68, Issue 38
****************************************

------------------------------

Message: 6
Date: Wed, 29 Jul 2009 15:13:03 -0400
From: Jimmy Markum <histoman3000 <@t> live.com>
Subject: RE: [Histonet] SLIDE PRINTER
To: <mitchellja <@t> email.chop.edu>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT109-W59E820FC4E189F06DEC49EAB120 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"

Janice,
I know this well!  It is a terrible item.  This was originally sold under AccuPlace and it barely worked, but showed very nice at various meetings under a very controlled environment.  In fact it worked so well, that the nice people at Thermo decided to purchase this from AccuPlace and more than double the price and hope to dupe us to purchase at almost 2700% increase for the same item.
While I have returned my units plenty of time and Thermo has added some of their "crack" engineering to the item.
Save your money and run from Thermo, buy a Leica or wait for TBS or someone else to come up with something better for a cost you can justify.
I will never purchase anything from Thermo again, every time you do, it only justifies what they are doing to the market.
Jimmy

> Date: Wed, 29 Jul 2009 11:42:27 -0400
> From: MITCHELLJA <@t> email.chop.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] SLIDE PRINTER
> 
> Anyone out there in Histoland have any experience with Thermo slide mate?  We are looking into purchasing a few.   Thanks for any input, Janice
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 7
Date: Wed, 29 Jul 2009 15:17:28 -0400
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] RE:Muscle Artifact
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<37E4BAC017F57141AF64FAA5AEB04CE8033A74B4 <@t> wlmmsx01.nemours.org>
Content-Type: text/plain; charset=iso-8859-1

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68, Issue 38

Send Histonet mailing list submissions to
	histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."

Today's Topics:

   1. Artifact in  Muscle bx's frozen for extended time (Sharon Allen)
   2. flash frozen tissue (Kalleberg, Kristopher)
   3. Mouse Eyes (Jo-Ann Bader, Ms.)
   4. SLIDE PRINTER (Janice Mitchell)
   5. Re: Mouse Eyes (Rene J Buesa)

----------------------------------------------------------------------

Message: 1
Date: Wed, 29 Jul 2009 09:27:24 -0500
From: "Sharon Allen" <SAllen <@t> exchange.hsc.mb.ca>
Subject: [Histonet] Artifact in  Muscle bx's frozen for extended time
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<BB6ADCD4B7ABB045A09A7634AC15CC610BEC8077 <@t> hscxmsmx0010.ad.wrha.mb.ca>
Content-Type: text/plain; charset="iso-8859-1"

Hi,
I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70°C freezer.  We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum,  I am assuming from drying out from months/years of freezing.
We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them.  We store them in a small plastic tube with a screw top.
I would like to know if anyone has further methods to eliminate this artefact.
Possibly sealing with OCT?  Placing water in the bottom of the tube?   Wrapping the muscle bx in Parafilm, tin foil etc?
What containers do you use to store them in if you do not get this artefact?
Suggestions for long term storage would be very much appreciated.
Thanks
Sharon Allen
Senior Neuropathology Technologist
HSC
WPG, MB, CA
This email and/or any documents in this transmission is intended for the
addressee(s) only and may contain legally privileged or confidential information.  Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.  If you receive this transmission in error, please notify the sender immediately and return the original.

Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original.

------------------------------

Message: 2
Date: Wed, 29 Jul 2009 10:31:39 -0400
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] flash frozen tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<0E6BC087F70F9C47ACFF2C203D6E329C067F7868 <@t> NTRSEVS30002.s3.ms.unilever.com>

Content-Type: text/plain;	charset="us-ascii"

Hello All,

I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen.  My big concern is what is the proper way to fix all of these SKIN samples to use for IHC.  Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best.  Any and and all help will be greatly appreciated.  Thank you.

Kris Kalleberg    

------------------------------

Message: 3
Date: Wed, 29 Jul 2009 11:38:05 -0400
From: "Jo-Ann Bader, Ms." <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<76D119EF12C904418800ED67CCB2062905E2AB9071 <@t> EXMBXVS1.campus.mcgill.ca>
Content-Type: text/plain; charset="us-ascii"

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6

------------------------------

Message: 4
Date: Wed, 29 Jul 2009 11:42:27 -0400
From: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>
Subject: [Histonet] SLIDE PRINTER
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A7035A3020000000052CB35 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII

Anyone out there in Histoland have any experience with Thermo slide mate?  We are looking into purchasing a few.   Thanks for any input, Janice

------------------------------

Message: 5
Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>,	" Ms.Jo-Ann Bader"
	<jo-ann.bader <@t> mcgill.ca>
Message-ID: <950812.3007.qm <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration.
René J.

--- On Wed, 7/29/09, Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca> wrote:

From: Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, July 29, 2009, 11:38 AM

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 68, Issue 38
****************************************

------------------------------

Message: 8
Date: Wed, 29 Jul 2009 15:19:11 -0400
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] RE: Muscle Artifact
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<37E4BAC017F57141AF64FAA5AEB04CE8033A74B5 <@t> wlmmsx01.nemours.org>
Content-Type: text/plain; charset=iso-8859-1

 Containers: Fisher # NC9283918 $32.90/6.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68, Issue 38

Send Histonet mailing list submissions to
	histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."

Today's Topics:

   1. Artifact in  Muscle bx's frozen for extended time (Sharon Allen)
   2. flash frozen tissue (Kalleberg, Kristopher)
   3. Mouse Eyes (Jo-Ann Bader, Ms.)
   4. SLIDE PRINTER (Janice Mitchell)
   5. Re: Mouse Eyes (Rene J Buesa)

----------------------------------------------------------------------

Message: 1
Date: Wed, 29 Jul 2009 09:27:24 -0500
From: "Sharon Allen" <SAllen <@t> exchange.hsc.mb.ca>
Subject: [Histonet] Artifact in  Muscle bx's frozen for extended time
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<BB6ADCD4B7ABB045A09A7634AC15CC610BEC8077 <@t> hscxmsmx0010.ad.wrha.mb.ca>
Content-Type: text/plain; charset="iso-8859-1"

Hi,
I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70°C freezer.  We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum,  I am assuming from drying out from months/years of freezing.
We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them.  We store them in a small plastic tube with a screw top.
I would like to know if anyone has further methods to eliminate this artefact.
Possibly sealing with OCT?  Placing water in the bottom of the tube?   Wrapping the muscle bx in Parafilm, tin foil etc?
What containers do you use to store them in if you do not get this artefact?
Suggestions for long term storage would be very much appreciated.
Thanks
Sharon Allen
Senior Neuropathology Technologist
HSC
WPG, MB, CA
This email and/or any documents in this transmission is intended for the
addressee(s) only and may contain legally privileged or confidential information.  Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.  If you receive this transmission in error, please notify the sender immediately and return the original.

Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original.

------------------------------

Message: 2
Date: Wed, 29 Jul 2009 10:31:39 -0400
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] flash frozen tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<0E6BC087F70F9C47ACFF2C203D6E329C067F7868 <@t> NTRSEVS30002.s3.ms.unilever.com>

Content-Type: text/plain;	charset="us-ascii"

Hello All,

I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen.  My big concern is what is the proper way to fix all of these SKIN samples to use for IHC.  Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best.  Any and and all help will be greatly appreciated.  Thank you.

Kris Kalleberg    

------------------------------

Message: 3
Date: Wed, 29 Jul 2009 11:38:05 -0400
From: "Jo-Ann Bader, Ms." <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<76D119EF12C904418800ED67CCB2062905E2AB9071 <@t> EXMBXVS1.campus.mcgill.ca>
Content-Type: text/plain; charset="us-ascii"

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6

------------------------------

Message: 4
Date: Wed, 29 Jul 2009 11:42:27 -0400
From: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>
Subject: [Histonet] SLIDE PRINTER
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A7035A3020000000052CB35 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII

Anyone out there in Histoland have any experience with Thermo slide mate?  We are looking into purchasing a few.   Thanks for any input, Janice

------------------------------

Message: 5
Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>,	" Ms.Jo-Ann Bader"
	<jo-ann.bader <@t> mcgill.ca>
Message-ID: <950812.3007.qm <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration.
René J.

--- On Wed, 7/29/09, Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca> wrote:

From: Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, July 29, 2009, 11:38 AM

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 68, Issue 38
****************************************

------------------------------

Message: 9
Date: Wed, 29 Jul 2009 15:22:01 -0400
From: Mike Valerio <valerio2 <@t> buffalo.edu>
Subject: [Histonet] Problems with IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <61151.1248895321 <@t> buffalo.edu>
Content-Type: text/plain; charset="utf-8"

Hello,
I am having problems staining my FFPE mouse brains for macrophages.  I started out with F4/80 
marker, but have now moved to CD68.  My positive controls (mouse spleen and liver) work fine, but 
I cannot get anything to stain in my sample tissues.  I have see through H and E staining that 
macrophages are present, could anyone help me with this problem?
Mike Valerio
University of Buffalo

------------------------------

Message: 10
Date: Wed, 29 Jul 2009 14:17:39 -0500
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Artifact in  Muscle bx's frozen for extended
	time
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<F2F030053F9B7345831BED293A6D57E109A71B <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>

Content-Type: text/plain;	charset="iso-8859-1"

We do everything you do, except we surround the tissue with oct in the tube before freezing. Otherwise, it's freezer burn city. (in TX I think) :)  We also just write the info on the side of the bottle with a thin sharpie instead of the cork thing. We usually store several tubes from the same case as we don't know how much tissue they will want in the future.
Claire

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Sharon Allen
Sent: Wed 7/29/2009 9:27 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Artifact in Muscle bx's frozen for extended time

Hi,
I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70°C freezer.  We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum,  I am assuming from drying out from months/years of freezing.
We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them.  We store them in a small plastic tube with a screw top.
I would like to know if anyone has further methods to eliminate this artefact.
Possibly sealing with OCT?  Placing water in the bottom of the tube?   Wrapping the muscle bx in Parafilm, tin foil etc?
What containers do you use to store them in if you do not get this artefact?
Suggestions for long term storage would be very much appreciated.
Thanks
Sharon Allen
Senior Neuropathology Technologist
HSC
WPG, MB, CA
This email and/or any documents in this transmission is intended for the
addressee(s) only and may contain legally privileged or confidential information.  Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.  If you receive this transmission in error, please notify the sender immediately and return the original.

Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 11
Date: Wed, 29 Jul 2009 15:26:27 -0400
From: "Justin Peters" <JPeters <@t> bostwicklaboratories.com>
Subject: [Histonet] Glycophorin A antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<24D22DE9E488AA43BF92A4389F2DDB1F064FB71C <@t> mail1.BOSTWICK.COM>
Content-Type: text/plain;	charset="us-ascii"

Has anyone had any luck with Glycophorin A antibody (Cell Marque) used
on paraffin-embedded (Formical decalcified) bone marrow biopsies?

Justin Peters, HTL (ASCP)

IHC Supervisor

Bostwick Laboratories(tm)
For Absolute Confidence(r)

4355 Innslake Drive
Glen Allen, Virginia 23060
Phone:    (804) 967-9225 ext. 1831
Cell:        (804) 822-6084
Email: jpeters <@t> bostwicklaboratories.com
<mailto:jpeters <@t> bostwicklaboratories.com>  

------------------------------

Message: 12
Date: Wed, 29 Jul 2009 14:30:00 -0500
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] flash frozen tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F2F030053F9B7345831BED293A6D57E109A71C <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>

Content-Type: text/plain;	charset="iso-8859-1"

We are a Mohs clinic and I had done a study on the feasability of IHC on frozen skin. I cut slides as normal then fixed in acetone before using Biocare's Mach 3 kit (Alk. phos. with Red chromogen) with predilute antibodies. They mostly turned out well, except for the S100. I don't believe that one turns out satisfactory in frozen tissue no matter what you do. I did a MART-1 stain, HMB-45 (predilute from Innovex), and Tyrosinase. There was very little background. Biocare will be more than happy to help with any questions you might have.  
Claire  

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Kalleberg, Kristopher
Sent: Wed 7/29/2009 9:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] flash frozen tissue

Hello All,

I will be running a study upcoming and we have decided not to run FFPE
as we normally do and have decided to use flash frozen.  My big concern
is what is the proper way to fix all of these SKIN samples to use for
IHC.  Any recommendations as to if prefix the samples in sucrose and
then flash freeze in OCT is best or any recommendations as to if post
fixing (in acetone??) the already sectioned, flash frozen sample in OCT
is best.  Any and and all help will be greatly appreciated.  Thank you.

Kris Kalleberg   
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 13
Date: Wed, 29 Jul 2009 14:31:20 -0500
From: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>
Subject: RE: [Histonet] RE: Muscle Artifact
To: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<CBAD8B8185C05346A5B74AE1E12CBF2A04BEB17E <@t> HC-MAIL11.healthcare.uiowa.edu>

Content-Type: text/plain;	charset="iso-8859-1"

This number did not work for me at the Fisher website.  Is it current?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barone, Carol 
Sent: Wednesday, July 29, 2009 2:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Muscle Artifact

 Containers: Fisher # NC9283918 $32.90/6.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68, Issue 38

Send Histonet mailing list submissions to
	histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."

Today's Topics:

   1. Artifact in  Muscle bx's frozen for extended time (Sharon Allen)
   2. flash frozen tissue (Kalleberg, Kristopher)
   3. Mouse Eyes (Jo-Ann Bader, Ms.)
   4. SLIDE PRINTER (Janice Mitchell)
   5. Re: Mouse Eyes (Rene J Buesa)

----------------------------------------------------------------------

Message: 1
Date: Wed, 29 Jul 2009 09:27:24 -0500
From: "Sharon Allen" <SAllen <@t> exchange.hsc.mb.ca>
Subject: [Histonet] Artifact in  Muscle bx's frozen for extended time
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<BB6ADCD4B7ABB045A09A7634AC15CC610BEC8077 <@t> hscxmsmx0010.ad.wrha.mb.ca>
Content-Type: text/plain; charset="iso-8859-1"

Hi,
I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70°C freezer.  We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum,  I am assuming from drying out from months/years of freezing.
We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them.  We store them in a small plastic tube with a screw top.
I would like to know if anyone has further methods to eliminate this artefact.
Possibly sealing with OCT?  Placing water in the bottom of the tube?   Wrapping the muscle bx in Parafilm, tin foil etc?
What containers do you use to store them in if you do not get this artefact?
Suggestions for long term storage would be very much appreciated.
Thanks
Sharon Allen
Senior Neuropathology Technologist
HSC
WPG, MB, CA
This email and/or any documents in this transmission is intended for the
addressee(s) only and may contain legally privileged or confidential information.  Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.  If you receive this transmission in error, please notify the sender immediately and return the original.

Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original.

------------------------------

Message: 2
Date: Wed, 29 Jul 2009 10:31:39 -0400
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] flash frozen tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<0E6BC087F70F9C47ACFF2C203D6E329C067F7868 <@t> NTRSEVS30002.s3.ms.unilever.com>

Content-Type: text/plain;	charset="us-ascii"

Hello All,

I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen.  My big concern is what is the proper way to fix all of these SKIN samples to use for IHC.  Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best.  Any and and all help will be greatly appreciated.  Thank you.

Kris Kalleberg    

------------------------------

Message: 3
Date: Wed, 29 Jul 2009 11:38:05 -0400
From: "Jo-Ann Bader, Ms." <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<76D119EF12C904418800ED67CCB2062905E2AB9071 <@t> EXMBXVS1.campus.mcgill.ca>
Content-Type: text/plain; charset="us-ascii"

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6

------------------------------

Message: 4
Date: Wed, 29 Jul 2009 11:42:27 -0400
From: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>
Subject: [Histonet] SLIDE PRINTER
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A7035A3020000000052CB35 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII

Anyone out there in Histoland have any experience with Thermo slide mate?  We are looking into purchasing a few.   Thanks for any input, Janice

------------------------------

Message: 5
Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>,	" Ms.Jo-Ann Bader"
	<jo-ann.bader <@t> mcgill.ca>
Message-ID: <950812.3007.qm <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration.
René J.

--- On Wed, 7/29/09, Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca> wrote:

From: Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, July 29, 2009, 11:38 AM

We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 68, Issue 38
****************************************

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 14
Date: Wed, 29 Jul 2009 13:56:14 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] slide and cassette labelers
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <23735D5B905B49A69DA0245AA96B4026 <@t> Patsyoffice>
Content-Type: text/plain;	charset="us-ascii"

Does anyone have any experience with the Leica IPC cassette labeler and IPS
slide labeler or the Sukura Tissue Tek Auto-write systems.  We are setting
up a new lab and need labelers that will interface with windows operating
systems and do not have these experiences.

Best regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

------------------------------

Message: 15
Date: Wed, 29 Jul 2009 16:03:08 -0400
From: eric.halpern <@t> comphealth.com
Subject: [Histonet] Eric M. Halpern/CompHealth is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OF30125296.C3947BDB-ON85257602.006E2675-85257602.006E2676 <@t> comphealth.com>

Content-Type: text/plain; charset=US-ASCII

I will be out of the office starting  07/29/2009 and will not return until
08/03/2009.

I will out of the office on Vacation starting Monday 7/29 and will return back
to work on Monday 8/3 in my absence if you need immediated attention please
contact Kiersten Ferreira  the Operations Manager at 866-782-9029 ext 5842 or at
kiersten.ferreira <@t> comphealth.com.

Regards,

Eric

------------------------------

Message: 16
Date: Wed, 29 Jul 2009 13:36:47 -0700
From: Andrea Grantham <algranth <@t> email.arizona.edu>
Subject: Re: [Histonet] SLIDE PRINTER
Cc: HISTONET <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B2C38868-DC07-4A5D-A18F-3A1E190CF4AB <@t> email.arizona.edu>
Content-Type: text/plain;	charset=WINDOWS-1252;	format=flowed;
	delsp=yes

BUT...I have a small lab and can't justify a more expensive printer so  
we purchased this printer - in fact we probably purchased the last one  
that AccuPlace sold before Thermo got it.
I like it a lot and even though we have returned a couple of them we  
have no complaints. It does a nice job. We were lucky to get it at the  
AccuPlace price.

Andi

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello
P Please consider the environment before printing this email.

On Jul 29, 2009, at 12:13 PM, Jimmy Markum wrote:

>
> Janice,
> I know this well!  It is a terrible item.  This was originally sold  
> under AccuPlace and it barely worked, but showed very nice at  
> various meetings under a very controlled environment.  In fact it  
> worked so well, that the nice people at Thermo decided to purchase  
> this from AccuPlace and more than double the price and hope to dupe  
> us to purchase at almost 2700% increase for the same item.
> While I have returned my units plenty of time and Thermo has added  
> some of their "crack" engineering to the item.
> Save your money and run from Thermo, buy a Leica or wait for TBS or  
> someone else to come up with something better for a cost you can  
> justify.
> I will never purchase anything from Thermo again, every time you do,  
> it only justifies what they are doing to the market.
> Jimmy
>
>
>> Date: Wed, 29 Jul 2009 11:42:27 -0400
>> From: MITCHELLJA <@t> email.chop.edu
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] SLIDE PRINTER
>>
>> Anyone out there in Histoland have any experience with Thermo slide  
>> mate?  We are looking into purchasing a few.   Thanks for any  
>> input, Janice
>>
>>
>>
>> _______________________________________________
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>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _________________________________________________________________
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> how.
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------------------------------

Message: 17
Date: Wed, 29 Jul 2009 14:05:29 -0700 (PDT)
From: "Nicholas David Evans" <ndevans <@t> stanford.edu>
Subject: [HISTONET] Antigen retrieval paraffin skin samples
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002c01ca1090$4d32f280$ce1d41ab <@t> DellDesktop2>
Content-Type: text/plain;	charset="US-ASCII"

Dear all,

Might anyone be able to offer their advice on the best method to retrieve
antigens on paraffin embedded skin sections? I am immunostaining for
epithelial cytokeratins, and I have block-to-block variation in the
quality of the staining - some block give consistently good staining while
others giving negligible staining. The tissues were prepared by fixation
in 4% PFA for 24 - 48 hours followed by dehydration and embedding. I
currently use 15 mins Ficin digestion. I suspect that the differences may
be accounted for by variability in the length of time samples were fixed
in PFA and then 70% ethanol.

Best wishes

Nick

------------------------------

Message: 18
Date: Wed, 29 Jul 2009 20:15:00 -0400
From: njoydobro <@t> aol.com
Subject: Re: [Histonet] urine cytology with red blood cells
To: Tina.Hayes <@t> va.gov, Histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CBDEC1E9D0309D-12B8-BA1 <@t> webmail-mh11.sysops.aol.com>
Content-Type: text/plain; charset="utf-8"

try this:

BD CytoRichâ"¢ Blue Preservative* 

General Purpose Cell Preservative.

Excellent for Urine and non-hemolytic samples.

Recommended for use as a general cytology preservative

Gene

-----Original Message-----
From: Hayes, Tina J. <Tina.Hayes <@t> va.gov>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, Jul 29, 2009 1:54 pm
Subject: [Histonet] urine cytology with red blood cells

Our pathologist would like us to set up a procedure for cytologies
urines, in particular) that will NOT lyse red blood cells.  Does
nybody know of a transport/preservation medium for urine cytologies
hat does not lyse RBC's?  And is anybody using it with ThinPrep?  We
re currently using ThinPrep with Cytolyt, however the Cytolyt solution
s designed to lyse the RBC's.
_______________________________________________
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istonet <@t> lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 19
Date: Thu, 30 Jul 2009 11:27:33 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Artifact in  Muscle bx's frozen for extended
	time
To: "Sharon Allen" <SAllen <@t> exchange.hsc.mb.ca>,
	<histonet <@t> pathology.swmed.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB0410 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="iso-8859-1"

Sharon,

Your answer is probably in the following article in the Journal of Histotechnology (Volume 32, number 2, June 2009):

"Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic Activity and Morphology"
Authors: Iren Horkayne-Szakaly, Glenn D. Sandberg, Joren Keylock, Elisabeth J. Rushing

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon Allen
Sent: Thursday, 30 July 2009 12:27 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Artifact in Muscle bx's frozen for extended time

Hi, 
I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70°C freezer.  We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum,  I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them.  We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact.
Possibly sealing with OCT?  Placing water in the bottom of the tube?   Wrapping the muscle bx in Parafilm, tin foil etc?
What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the
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End of Histonet Digest, Vol 68, Issue 39
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