[Histonet] IHC on stored BALf cytospins

[Luke Garratt]

Hello all,

 

We have a large resource of unfixed cytospun glass slides of human bronchoalveolar lavage fluid (BALf) that we would like to use for immunocytochemistry. For each BAL we have one pair of slides that are air-dried and then stored at RT, these date back up to 2001. We also have pairs of slides that were directly frozen at -20°C dating back to early 2006. Although we have generally used one of the air-dried slides for differential counts with Leishman's stain, the other 3 have remained in storage.

 

Having searched the literature for advice on how long a slide can be stored before it is used for ICC, to no avail, can somebody please give us some guidance on a) which slide is best to use (dried or frozen), b) the upper limit of storage, c) any special treatment the older slides may need and d) any recommended literature.

 

Many thanks,

 

Luke Garratt

Research Assistant

 

Australian Respiratory Early Surveillance Team for Cystic Fibrosis

Telethon Institute for Child Health Research

P.O. Box 855, West Perth, Western Australia, 6872

 

T  +61 8 9489 7819          F  +61 8 9489 7700

W www.arestcf.org <http://www.arestcf.org>            E  lgarratt <@t> ichr.uwa.edu.au <mailto:lgarratt <@t> ichr.uwa.edu.au> 

 

 


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