[Histonet] Start Up Lab

Thu Jul 23 12:40:43 CDT 2009

I went to the Navy's MLT program in 79-80 and we did drug screens manually and the chemicals that we used would give us a buzz. Thought it was ironic that I was getting high while looking for drugs in somebody else.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello [vapatpxs <@t> yahoo.com]
Sent: Thursday, July 23, 2009 1:30 PM
To: Lynette Pavelich; HistoNet
Subject: RE: [Histonet] Start Up Lab

In the 80's I worked in a drug screening lab for the Navy and one step in the extraction involved fuming HCl (meaning pure HCl).  Several of the techs got nose bleeds every time they used the stuff.  I finally pried open a window (they were painted shut) and we turned on a fan the size of a propeller to blow the fumes out the window.

We used Xylene in a room with no ventilation and found out that high concentrations of xylene cause "transient euphoria".  No wonder we thought every thing was funny during those days.

Safety people, they take all the fun out of research!  ;-)

Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF)

Core Research Imaging Center (CRIC)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397

Your images flow through our CRIC.

--- On Thu, 7/23/09, Lynette Pavelich <lpaveli1 <@t> hurleymc.com> wrote:

From: Lynette Pavelich <lpaveli1 <@t> hurleymc.com>
Subject: RE: [Histonet] Start Up Lab
To: cbrya <@t> lexclin.com, Montina.VanMeter <@t> pbrc.edu, thecitan <@t> yahoo.com
Cc: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, July 23, 2009, 3:53 PM

Ah, yes.  The '70's.  Our lab had 2 windows, both on the same side of
the wall.  The pathologists cut under one of the windows and it had one
of those box fans, pointing out.  In Michigan.  No hoods, an old
duo-autotechnicon and xylene in full use with disposal down the drain.
Every day, I would go home not knowing if I went thru a red light or
not, arriving home , head still cloudy for about 30 minutes until it
cleared.  Ah..those were the days??  Although I miss it, I'm glad I had
to give up my coffee and donut when cutting.  Treats in the drawer are
gone, mouth pipetting is gone (not mine, but yes, the mouth does turn
black from silver nitrate!!)  Progress is good.  But I still like my
'70's music!!!  Bob Seger and old motown!!! ;)

>>> "Montina Van Meter" <Montina.VanMeter <@t> pbrc.edu> 07/23/09 11:31 AM
>>>
I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their "personal drawer".  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thecitan <@t> yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>

Date: Wed, 22 Jul 2009 18:07:07
To: <leiker <@t> buffalo.edu>; <histonet <@t> lists.utsouthwestern.edu>;
<CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Start Up Lab

Gosh.........I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh......

>>> Merced M Leiker <leiker <@t> buffalo.edu> 07/22/09 5:00 PM >>>
(lol some labs have a bench area as well as a desk area where food is
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire
<CIngles <@t> uwhealth.org> wrote:

> In the lab?!? For shame. :)
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Cindy
DuBois
> Sent: Wed 7/22/2009 10:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Start Up Lab
>
>
>
> And a coffee pot.
>
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.

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