[Histonet] 100 micron sections

[kbowden]

Yes, with H&E staining.  They think they need a thick section because 
they are looking for product that is 75mm round.  I don't seem to be 
able to make them understand that it is not necessary to cut that thick 
to see the beads they are looking for.
*/--
Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
858-534-4655 voice
858-534-5304 fax


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Barbara Albert wrote:
> Karen,
> Are you certain that they want these sections on slides?  The thicker 
> ones we usually put into a microcentrifuge tube.  No water bath, and 
> the curled section fits into the tube.
>
> Barbara Albert
> San Francisco
>
> ------------------------------------------------------------------------
> *From:* kbowden <kbowden <@t> ucsd.edu>
> *Cc:* histonet <histonet <@t> lists.utsouthwestern.edu>
> *Sent:* Wednesday, July 22, 2009 4:46:31 PM
> *Subject:* [Histonet] 100 micron sections
>
> Hi,
>
> I am being asked to section paraffin embedded fat at 100 microns.  I 
> haven't sectioned anything in paraffin thicker than 20 microns.  What 
> are the tricks to get it to unroll in the water bath? -- */--
> Karen Bowden
> Staff Research Associate II
> University of CA, San Diego
> Department of Orthopedics
> 9500 Gilman Dr. 0630
> La Jolla, CA 92093-0630
> 858-534-4655 voice
> 858-534-5304 fax
>
>
> CONFIDENTIALITY NOTICE:
> THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE
> PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL
> AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR
> OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION
> BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED.
> IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND
> DELETE THE MATERIAL FROM ANY COMPUTER./*
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