[Histonet] Celloidin Sections.

[JR R]

Hi Barry,

I am studying the development of atherosclerotic lesions in mouse brachiocephalic artery--I typically use 5 micron sections.   Paraffin sections have given me mostly good, frequently excellent and occasionally spectacular results, morphology-wise.  Oh, and occasionally poor results, too.  I would prefer to increase the ration of spectacular/merely OK results.  

I read an article where the authors describe getting better fine morphology with celloidin than paraffin.

Atheromas, like cochlea are delicate structures with fine detail, and they can easily become detached from the artery wall.  After reading the article, I can't help but wonder if a different embedding media might give me even better results than I am already getting.

Would I need a special microtome for celloidin?  One reason I don't want to go with methacrylate is I don't have budget for new equipment right now.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology




Effects of Fixative and Embedding
Medium on Morphology and

Immunostaining
of the Cochlea



 Audiol Neurootol. 2009 ; 14(2): 78–87





> From: Barry.R.Rittman <@t> uth.tmc.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 20 Jul 2009 19:14:58 -0500
> Subject: RE: [Histonet] Celloidin Sections.
> 
> Jerry
> depends on whether you are talking about celloidin or Low Viscosity Nitrocelulose.
> There are techniques in the literature to cut celloidin sections as thin  as 4 microns.
> For LVN you need much thicker sections as this plastic is much less sturdy and has a tendency to fragment if too thin.
> The questions I ask is why you need celloidin sections and what tissue are you thinking about for this technique?
> Barry
> 
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of JR R [rosenfeldtek <@t> hotmail.com]
> Sent: Monday, July 20, 2009 6:42 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Celloidin Sections.
> 
> I am curious--How thin can celloidin sections be cut?  I've heard that 30 microns is a standard thickness.  No way to make 5 micron sections?
> 
> Thanks,
> 
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
> 
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