[Histonet] Celloidin Sections.

[Rittman, Barry R]

Jerry
depends on whether you are talking about celloidin or Low Viscosity Nitrocelulose.
There are techniques in the literature to cut celloidin sections as thin  as 4 microns.
For LVN you need much thicker sections as this plastic is much less sturdy and has a tendency to fragment if too thin.
The questions I ask is why you need celloidin sections and what tissue are you thinking about for this technique?
Barry

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of JR R [rosenfeldtek <@t> hotmail.com]
Sent: Monday, July 20, 2009 6:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Celloidin Sections.

I am curious--How thin can celloidin sections be cut?  I've heard that 30 microns is a standard thickness.  No way to make 5 micron sections?

Thanks,


Jerry Ricks
Research Scientist
University of Washington
Department of Pathology

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