[Histonet] Re: Leica Autostainer Problems

[Asquith Stephen]

Hi All
Many thanks for the posts that were sent regarding our problem. The
local Leica agents in John and Marina came out and checked out the
machine. The next day Marina spent the day with us trouble shooting with
a perfect H&E as an end result. The problem was sloved by including a
wash step prior to the dehydration steps after the Eosin. This ensured
all the eosin was removed from the slide prior to the dehydration steps.
Droplets of eosin were being left on the lable portion of the slides and
these then dripped down the slide removing some of the eosin stain and
hence our problem.
Happy histologists all round. Many thanks to Marina

Stephen Asquith

Microscopy Imaging and Research Core Facility
Peter MacCallum Cancer Centre
Locked Bag #1
A'Beckett Street
East Melbourne  8006

Email:  stephen.asquith <@t> petermac.org
Phone +61-3-9656 1244/1243
Fax +61-3-9656 1411




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