[Histonet] 4F:1G

Adam adamcpeer <@t> gmail.com
Thu Jul 2 17:06:06 CDT 2009


I'm new to the field of histology and want to use 4F:1G to fix fish
ovaries for later histological examination under light microscopy.
Following fixation, I plan to rinse the ovary samples in an ethanol
series (50-95%).  First, what is the minimum time needed to fix 10 g
of tissue?  Second, I would like to store the fixed samples for a few
months prior to embedding.  Is it best to maintain them in 4F:1G or to
keep them in ethanol after the rinses?



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