[Histonet] osteoids

[Jack Ratliff]

My recommendation to you is to focus on your undemineralized bone  
sections embedded in MMA. These sections will provide you with the  
most information.

If you do a Goldners stain you can very accurately measure BV/TV and  
clearly quantitate osteoid volume. Bone will stain green from the SF  
yellowish staining and osteoid red with the acid fuchsin/ponceau. I  
feel the only limitation with this stain is clearly assessing your  
bone cells.

If you stain with a Von Kossa/MacNeal's tetrachrome you can achieve  
the above (bone = black, osteoid = greyish-green) and you can also  
clearly assess osteoblast (blue) activity. This stain also helps to  
identify osteoclast presence but hard to accurately determine activity  
without a TRAP stain follow up.

Write me back with any additional questions you may have and when I  
get to the lab I will forward you a couple of protocols and additional  
information!

Best,

Jack

Sent from my iPhone

On Jul 2, 2009, at 7:59 AM, "Manav Mehta" <manav.mehta <@t> charite.de>  
wrote:

> Dear Histonet'ers,
> I am trying to stain for Osteoids in a fracture callus of rat  
> femurs. I
> have MMA and Paraffin sections (4-5 um) / blocks already made.  
> However,
> I am not sure what stain I should use. Peers have suggested that a  
> Movat
> pentachrome should work, while some have suggested Goldner, and silver
> staining. I am not sure how to proceed at the moment. I would  
> appreciate
> any suggestions with possibly a protocol on the staining for osteoids.
> It would also help if you provided references on this stain or details
> on the stain. Life experiences while working with these stains are  
> also
> welcome.
>
> The Goal is to characterize the osteoids in the callus (surface,
> thickness, osteoblast numbers). Possibly relate them to osteoblastic  
> and
> -clastic activity.
>
> Thanks,
> Manav Mehta
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