[Histonet] Re: Eosin in alcohol

[Blazek, Linda]

We use about the same amount or eosin in the first 100% alcohol and also do immunos on the biopsies and have no problem.  We do all GI biopsies though not prostate.  I'm not sure why the eosin would interfere with the immuno staining since it disappears during the hydration and retrieval process of staining immunos.    


 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson
Sent: Wednesday, July 01, 2009 1:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Eosin in alcohol


We have used Eosin in the last 95% alcohol on the tissue processor for several years.  I usually add approximately 5 ml to the full jug.  It is a great tool to use for embedding.  However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining.  They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue.  There is no delineations in the color of the blue and I found it to be useless for helping to embed.  I would rather do without anything than use cobalt blue.  I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies.  

Thanks,

Jennifer Johnson, HTL (ASCP) 

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