[Histonet] Re: Eosin in alcohol

[DKBoyd <@t> chs.net]

We use eosin on the gross bench.  We keep a bottle of concentrated Eosin 
and Hematoxylin on the bench with an eyedropper.  The eosin is for 
endoscopic biopsies and the hematoxylin is for prostate biopsies.  Neither 
has never interfered with IHC staining.

Debbie M. Boyd, HT(ASCP),Chief Histologist, Southside Regional Medical 
Center, 
200 Medical Park Boulevard, Petersburg, Va.  23805, T: 804-765-5050, F: 
804-765-5582, dkboyd <@t> chs.net







Jennifer Johnson <jmjohnson34 <@t> hotmail.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/01/2009 01:09 PM

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Subject
[Histonet] Re:  Eosin in alcohol







We have used Eosin in the last 95% alcohol on the tissue processor for 
several years.  I usually add approximately 5 ml to the full jug.  It is a 
great tool to use for embedding.  However, we received a letter from the 
lab that we send our prostate biopsies to saying that it was undesirable 
because it interfered with their immuno staining.  They sent us some 
cobalt blue to use in the place of eosin along with mixing instructions 
and the whole batch of tissues came out such a dark blue.  There is no 
delineations in the color of the blue and I found it to be useless for 
helping to embed.  I would rather do without anything than use cobalt 
blue.  I guess the point of my rambling is, Eosin is a wonderful tool to 
use unless you are doing immunos on prostate biopsies. 

Thanks,

Jennifer Johnson, HTL (ASCP) 

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