[Histonet] Need help for CD95(FAS) and antigen retrieval system

[Patsy Ruegg]

What detection system are you using?

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org    
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of soofia
siddiqui
Sent: Friday, October 24, 2008 12:10 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Need help for CD95(FAS) and antigen retrieval system

Hi everyone,
I am sure some one in this large world of histonet will be able to help me.
I am trying to do CD95 staining on human skin FF PE tissue sections. CD95 is
45 KD single chain type cell surface  protein that mediates apoptosis when
crosslinked with agnostic anti-Fas antibodies.I have tried citric acid pH
6.0 buffer in microwave and and 99 degree Celsius water bath incubator and
also tried sodium citrate Buffer pH 6.00 on microwave and incubator and
also Trypsin for antigen retrieval, But I could not get any success. I am
using R&D MAB 142 monoclonal antibody Clone DX2 and also using BioLegend
3056130 monoclonal antibody clone DX2, but none of the method worked to get
the signaling. Please provide me, if   you  have some suggestion or any
better antigen retrieval method for Fas antibodies. Thank you in advance. 
Soofia
UWHC,Madison, WI 


      
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