[Histonet] RE: [IHCRG] MAOB IHC on either frozen or FFPE sections

[Merced Leiker]

...considering the stock Ab concentration isn't even known, as Brett 
mentioned.

--On Friday, October 24, 2008 1:09 PM -0400 Merced Leiker 
<leiker <@t> buffalo.edu> wrote:

>
> 3ug/ml sounds awful low for a stock concentration...
>
>
> --On Friday, October 24, 2008 11:51 AM -0500 "Swain, Frances L"
> <SwainFrancesL <@t> uams.edu> wrote:
>
>> Hi bert ifyou will look at the image for the antibody for IHC of MAOB you
>> will see at the bottom that the antibody demonstrated was at a
>> concentration of 3 ug/ml
>>
>>
>>
>> Frances L. Swain HT(ASCP) A. A. S.
>>
>> Special Procedures Technician
>>
>> Department of Orthopaedic Surgery
>>
>> Center for Orthopaedic Research
>>
>> Barton Research Building 2R28
>>
>> 4301 West Markham Street
>>
>> Little Rock AR 72205
>>
>> (501) 686-8739 PHONE
>>
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>>
>> swainfrancesl <@t> uams.edu email
>>
>> ________________________________
>>
>> From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
>> Connolly, Brett M
>> Sent: Friday, October 24, 2008 10:41 AM
>> To: ihcrg <@t> googlegroups.com; histonet <@t> lists.utsouthwestern.edu
>> Subject: [IHCRG] MAOB IHC on either frozen or FFPE sections
>>
>>
>>
>> Has anyone done it? I have an antibody from Abnova. They illustrate
>> staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab
>> concentration is they say they never determined that. Go figure? I'm
>> looking for a starting point dilution.
>>
>> Thanks,
>> Brett
>>
>> Brett M. Connolly, Ph.D.
>> Research Fellow, Imaging Research
>> Merck & Co., Inc.
>> PO Box 4, WP-44K
>> West Point, PA 19486
>> PH 215-652-2501 fax. 215-993-6803
>> e-mail. brett_connolly <@t> merck.com
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>
>
> Merced M Leiker
> Research Technician II
> 354 BRB (packages) / 140 Farber Hall (mail)
> School of Medicine and Biomedical Sciences
> State University of New York at Buffalo
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Merced M Leiker
Research Technician II
354 BRB (packages) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

"Without my flaws I'm really very boring."
- random internet blog commentator