[Histonet] RE: [IHCRG] MAOB IHC on either frozen or FFPE sections

[Merced Leiker]

3ug/ml sounds awful low for a stock concentration...


--On Friday, October 24, 2008 11:51 AM -0500 "Swain, Frances L" 
<SwainFrancesL <@t> uams.edu> wrote:

> Hi bert ifyou will look at the image for the antibody for IHC of MAOB you
> will see at the bottom that the antibody demonstrated was at a
> concentration of 3 ug/ml
>
>
>
> Frances L. Swain HT(ASCP) A. A. S.
>
> Special Procedures Technician
>
> Department of Orthopaedic Surgery
>
> Center for Orthopaedic Research
>
> Barton Research Building 2R28
>
> 4301 West Markham Street
>
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>
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> swainfrancesl <@t> uams.edu email
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> ________________________________
>
> From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
> Connolly, Brett M
> Sent: Friday, October 24, 2008 10:41 AM
> To: ihcrg <@t> googlegroups.com; histonet <@t> lists.utsouthwestern.edu
> Subject: [IHCRG] MAOB IHC on either frozen or FFPE sections
>
>
>
> Has anyone done it? I have an antibody from Abnova. They illustrate
> staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab
> concentration is they say they never determined that. Go figure? I'm
> looking for a starting point dilution.
>
> Thanks,
> Brett
>
> Brett M. Connolly, Ph.D.
> Research Fellow, Imaging Research
> Merck & Co., Inc.
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Merced M Leiker
Research Technician II
354 BRB (packages) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
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