[Histonet] Baker's Fluid
Jo-Ann Bader, Ms.
jo-ann.bader <@t> mcgill.ca
Mon Oct 20 10:34:56 CDT 2008
Thanks that is the one I am looking for!
Jo-Ann Bader
Histology Facility Coordinator
McGill Cancer Center
Cancer Pavillion
1160 Pine Ave W
Rm. 3355
Montreal, QC,
Tel: 514-398-8270
Fax: 514-8398-6769
email: jo-ann.bader <@t> mcgill.ca
-----Original Message-----
From: Weems, Joyce [mailto:JWeems <@t> sjha.org]
Sent: Mon 10/20/2008 11:16 AM
To: Jo-Ann Bader, Ms.; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Baker's Fluid
This was in the archives. Hope it helps!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
678-843-7831 - Fax
On Wed, 24 May 2000, Barry Rittman wrote:
> what you need is "Mollifex" sold by BDH
You shouldn't use secret mixtures, but Mollifex
isn't (or isn't quite) a secret formulation. BDH
made it following a publication by J. R. Baker
(1941) A fluid for softening tissues embedded in
paraffin wax. Quart. J. Microsc. Sci. 61:75.
Bakers experiments led him to the composition:
Glycerol 10
60% ethyl alcohol: 90
(parts by volume).
A more recent paper is by Maria Wynnchuk (1992)
in J. Histotechnol. 15(4): 321-323. She used
a mixture called Horne's modification of Baker's
solution:
95% ethanol 9520 ml
Glycerol 320 ml
Acetone 80 ml
Liquid phenol 80 ml
(I guess that the "liquid phenol" is an 80%
solution in water, not solid phenol that has
been melted by heating.) 10 litres of softening
solution seems a lot to make up! I wonder
how long it lasts for.
Wynnchuk states that this mixture is better
than Baker's original and that Mollifex, which
contains a "phenol derivative" is just as good.
Anything here for Messrs Sue, Grabbit and Runne?
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jo-Ann
Bader, Ms.
Sent: Monday, October 20, 2008 10:42 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Baker's Fluid
Does anyone have the recepe fpr Baker's fluid?
Jo-Ann Bader
Histology Facility Coordinator
McGill Cancer Center
Cancer Pavillion
1160 Pine Ave W
Rm. 3355
Montreal, QC,
Tel: 514-398-8270
Fax: 514-8398-6769
email: jo-ann.bader <@t> mcgill.ca
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