[Histonet] Baker's Fluid

[Weems, Joyce]

This was in the archives. Hope it helps! 

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
678-843-7831 - Fax




On Wed, 24 May 2000, Barry Rittman wrote:

> what you need is "Mollifex" sold by BDH

  You shouldn't use secret mixtures, but Mollifex
  isn't (or isn't quite) a secret formulation. BDH
  made it following a publication by J. R. Baker
  (1941) A fluid for softening tissues embedded in
  paraffin wax. Quart. J. Microsc. Sci. 61:75.
  Bakers experiments led him to the composition:
      Glycerol           10
      60% ethyl alcohol: 90
         (parts by volume).
  A more recent paper is by Maria Wynnchuk (1992)
  in J. Histotechnol. 15(4): 321-323. She used
  a mixture called Horne's modification of Baker's
  solution:
     95% ethanol       9520 ml
     Glycerol           320 ml
     Acetone             80 ml
     Liquid phenol       80 ml
  (I guess that the "liquid phenol" is an 80%
   solution in water, not solid phenol that has 
   been melted by heating.) 10 litres of softening
   solution seems a lot to make up! I wonder
   how long it lasts for.

  Wynnchuk states that this mixture is better
  than Baker's original and that Mollifex, which
  contains a "phenol derivative" is just as good. 
  Anything here for Messrs Sue, Grabbit and Runne?

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jo-Ann
Bader, Ms.
Sent: Monday, October 20, 2008 10:42 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Baker's Fluid

Does anyone have the recepe fpr Baker's fluid?

Jo-Ann Bader
Histology Facility Coordinator
McGill Cancer Center
Cancer Pavillion
1160 Pine Ave W
Rm. 3355
Montreal, QC,
Tel: 514-398-8270
Fax: 514-8398-6769
email: jo-ann.bader <@t> mcgill.ca



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