[Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa

[Jane M Flinn]

We are running a congo red stain and have sodium chloride crystals precipataing onto the slide. Does anyone know why and how we can fix this? jane

"Life is short - make haste to be kind"

Dr. Jane Flinn
Director, Undergraduate Neuroscience Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA  22030
Phone: 703-993-4107
Fax: 703-993-1359


----- Original Message -----
From: kbowden <kbowden <@t> ucsd.edu>
Date: Wednesday, October 8, 2008 5:37 pm
Subject: Re: [Histonet] citrisolve?

> I have used Citrisolve for serveral years.  I don't think anything 
> compares to Xylene, but I do think this comes very close.  I 
> change it 
> about once a week.  It pretty much deparaffinizes in the same 
> amount of 
> time.  Disposal is dependent on the regulations in your area.  
> When I 
> started using it our Environmental Health and Safety said pour it 
> down 
> the drain, but as time goes by and with more regulations now I 
> have to 
> collect it and have it removed with hazardous waste.
> --
> Karen Bowden
> Staff Research Associate II
> University of CA, San Diego
> Department of Orthopedics
> 9500 Gilman Dr. 0630
> La Jolla, CA 92093-0630
> 858-534-4655 voice
> 858-534-5304 fax
> 
> 
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> 
> Patten, Nicole (NIH/NIAAA) [F] wrote:
> > Does anyone have any experience using Citrisolve as a Xylene 
> alternative> to deparaffinize tissue sections? Do I use it just 
> like Xylene for the
> > same amount of time? Can it be reused or should I use new Citrisolve
> > each time? How do I dispose of it? 
> >
> >  
> >
> > Any help at all would be much appreciated. Thanks!
> >
> >  
> >
> > Nicole J. Patten
> > Post-Baccalaureate Fellow/IRTA
> > National Institutes of Health
> >
> >  
> >
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