[Histonet] citrisolve?

[Rene J Buesa]

Nicole:
CitriSolve works somewhat better than other d-Limonene xylene substitutes because it is a hybrid.
It contains d-Limonene at 70% (or 50% according with other recipes) + quaternary amnine mixture (or aliphatic hydrocarbons in another recipe) at 25% (or greater %) and 2-butoxyethanol at 5%. This 2-butoxyethanol is a suspected carcinogen with a TWA of only 25 ppm.
CitriSolve is described as corrosive to the eyes. It costs 2.31 times more than Xylene and
I personally would not use it.
René J. 

--- On Wed, 10/8/08, kbowden <kbowden <@t> ucsd.edu> wrote:

From: kbowden <kbowden <@t> ucsd.edu>
Subject: Re: [Histonet] citrisolve?
To: "Patten, Nicole (NIH/NIAAA) [F]" <pattennj <@t> mail.nih.gov>
Cc: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, October 8, 2008, 5:37 PM

I have used Citrisolve for serveral years.  I don't think anything 
compares to Xylene, but I do think this comes very close.  I change it 
about once a week.  It pretty much deparaffinizes in the same amount of 
time.  Disposal is dependent on the regulations in your area.  When I 
started using it our Environmental Health and Safety said pour it down 
the drain, but as time goes by and with more regulations now I have to 
collect it and have it removed with hazardous waste.
--
Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
858-534-4655 voice
858-534-5304 fax


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Patten, Nicole (NIH/NIAAA) [F] wrote:
> Does anyone have any experience using Citrisolve as a Xylene alternative
> to deparaffinize tissue sections? Do I use it just like Xylene for the
> same amount of time? Can it be reused or should I use new Citrisolve
> each time? How do I dispose of it? 
>
>  
>
> Any help at all would be much appreciated. Thanks!
>
>  
>
> Nicole J. Patten
> Post-Baccalaureate Fellow/IRTA
> National Institutes of Health
>
>  
>
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>   
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