[Histonet] Decolorize for IHC

[Dawson, Glen]

I just did a CK20 on an H&E and Linda is correct, there is no need to decolorize before doing the CK20.  The only thing I would suggest is that you are sure that all mounting media is removed after the coverslip comes off your H&E.  The longer it's been coverslipped the more mindful of mounting media removal you should be.

Good Luck,
Glen Dawson
IHC Manager
Milwaukee, WI


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sebree
Linda A.
Sent: Monday, October 13, 2008 2:47 PM
To: Paula Lucas; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Decolorize for IHC


We don't bother decolorizing H&Es since the IHC methodology generally
takes out the histochemical staining....also, we're counterstaining with
hematoxylin anyway.  You should have no problem doing CK20 over an H&E.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Monday, October 13, 2008 2:38 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Decolorize for IHC

Hello,

I need to decolorize an H&E stained section for Cytokeratin 20 and I
would like to know the proper way of decolorizing for IHC.  Is it even
possible to get a CK 20 positive stain after doing so?

Thanks,
Paula Lucas HT
Lab Manager
Bio-Path Medical Group

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