[Histonet] Decolorize for IHC

Sebree Linda A. LSebree <@t> uwhealth.org
Mon Oct 13 14:47:10 CDT 2008


We don't bother decolorizing H&Es since the IHC methodology generally
takes out the histochemical staining....also, we're counterstaining with
hematoxylin anyway.  You should have no problem doing CK20 over an H&E.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Monday, October 13, 2008 2:38 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Decolorize for IHC

Hello,

I need to decolorize an H&E stained section for Cytokeratin 20 and I
would like to know the proper way of decolorizing for IHC.  Is it even
possible to get a CK 20 positive stain after doing so?

Thanks,
Paula Lucas HT
Lab Manager
Bio-Path Medical Group

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