[Histonet] restarting DAB reaction

[Rene J Buesa]

I also do not see any reason why you cannot hydrate the sections again and add the DAB. It should work.
René J.

--- On Mon, 10/13/08, Emily Sours <talulahgosh <@t> gmail.com> wrote:

From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: [Histonet] restarting DAB reaction
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, October 13, 2008, 1:17 PM

Has anyone ever tried to continue a DAB reaction after the slides have been
washed and coverslipped?
I can't think of a reason why this wouldn't work, since we just wash
the
slides in PBS and water after the DAB step.
We use a vectastain kit for this--would we need to add more of the ABC
reagent (after removing the coverslips and washing in PBS) and then do DAB?

Emily
-- 
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