[Histonet] restarting DAB reaction

[Emily Sours]

Has anyone ever tried to continue a DAB reaction after the slides have been
washed and coverslipped?
I can't think of a reason why this wouldn't work, since we just wash the
slides in PBS and water after the DAB step.
We use a vectastain kit for this--would we need to add more of the ABC
reagent (after removing the coverslips and washing in PBS) and then do DAB?

Emily
-- 
the velocity of time turns her voice into sugar water
http://www.youtube.com/watch?v=jNA6zzoObxg