[Histonet] Richard Allan Hematoxylin problems

[Angela Bitting]

We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now.

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
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>>> "Gayle Callis" <gayle.callis <@t> bresnan.net> 5/20/2008 4:00 PM >>>
We use xylene to remove coverslips and do this work inside a hood.  After 
coverslip is off and mounting media is gone with an extra xylene rinse, we 
go back to Clearite 3 to rinse off xylene and recoverslip.  Anything to try 
an minimize exposure to xylene is done.  Unfortunately, we found the 
substitute just didn't work for this job.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT


----- Original Message ----- 
From: "Patty Dunlop" <pdunlop720 <@t> gmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, May 20, 2008 1:20 PM
Subject: [Histonet] xylene substitute recoverslipping


> Hello Histonetters,
>
> I use Clear-Rite 3, a xylene substitute, and am having problems when I 
> need
> to recoverslip.  Currently, I just put the slide that needs recoverslipped
> into a coplin jar of Clear-rite 3 at room temperature.  The coverslip 
> really
> doesn't like to come off (takes a week) and it tends to be a sticky, messy
> process.  Does anyone have a method for removing coverslips with a xylene
> substitute?  Should I use heat?
>
> Thanks,
> Patty
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


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