[Histonet] xylene substitute recoverslipping

[Gayle Callis]

We use xylene to remove coverslips and do this work inside a hood.  After 
coverslip is off and mounting media is gone with an extra xylene rinse, we 
go back to Clearite 3 to rinse off xylene and recoverslip.  Anything to try 
an minimize exposure to xylene is done.  Unfortunately, we found the 
substitute just didn't work for this job.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT


----- Original Message ----- 
From: "Patty Dunlop" <pdunlop720 <@t> gmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, May 20, 2008 1:20 PM
Subject: [Histonet] xylene substitute recoverslipping


> Hello Histonetters,
>
> I use Clear-Rite 3, a xylene substitute, and am having problems when I 
> need
> to recoverslip.  Currently, I just put the slide that needs recoverslipped
> into a coplin jar of Clear-rite 3 at room temperature.  The coverslip 
> really
> doesn't like to come off (takes a week) and it tends to be a sticky, messy
> process.  Does anyone have a method for removing coverslips with a xylene
> substitute?  Should I use heat?
>
> Thanks,
> Patty
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