[Histonet] Microwave deparaffinization.
anthony <@t> histotechexchange.com
anthony <@t> histotechexchange.com
Mon May 19 22:18:42 CDT 2008
Dear all:
Paraffin can be heated by a microwave. Normal microwaves are set to create
a wavelength that affects water molecules. You need a microwave that
affects long carbon chain molecules.
Funny, I was talking to a chemist who teaches at our local University, and
I asked her about breaking down wax less complex carbon chain molecules.
She told me that you can but. Unfortunately, when not tightly regulated,
it is called a fire.
Talk about being flamed. Ha ha.
Yours truly,
Anthony Williams BSc. HT (ASCP)
Histotech Exchange LLC
19 Whitmore St.
Lexington, VA 24450
T 1 877 464 8911
T 1 877 GO HT 911
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anthony <@t> histotechexchange.com
www.histotechexchange.com
Paraffin is "transparent" or "translucent" to microwaves, meaning that
> paraffin CANNOT be melted or even heated using microwaves.
> Instruments using microwaves heat the container (made of or including
> parts made of WEFLON) that melt the paraffin by convection from the hot
> container but NOT because the paraffin is directly heated.
> This is one of those good ideas that will not fly!
> René J.
>
> Sebastián Vecchio <boreades <@t> gmail.com> wrote:
> I have been trying to deparafinize section in the lab, but I cannot get
> the
> right time and power for the MW oven, does any one has a protocol for this
> .
> This method eliminates the uses of xylol in the processing of staining
> sections.
> Thanks.
> Sebastian, Student of Histotechnology, EUTM, FMED, UDELAR.
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