[Histonet] Microwave deparaffinization.

Rene J Buesa rjbuesa <@t> yahoo.com
Mon May 19 15:48:42 CDT 2008


Paraffin is "transparent" or "translucent" to microwaves, meaning that paraffin CANNOT be melted or even heated using microwaves.
  Instruments using microwaves heat the container (made of or including parts made of WEFLON) that melt the paraffin by convection from the hot container but NOT because the paraffin is directly heated.
  This is one of those good ideas that will not fly!
René J.

Sebastián Vecchio <boreades <@t> gmail.com> wrote:
  I have been trying to deparafinize section in the lab, but I cannot get the
right time and power for the MW oven, does any one has a protocol for this
.
This method eliminates the uses of xylol in the processing of staining
sections.
Thanks.
Sebastian, Student of Histotechnology, EUTM, FMED, UDELAR.
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