[Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin)

[Geoff McAuliffe]

As a number of listers have mentioned, this is not a difficult technique.
That said, I always make my periodic acid fresh on the day of use.
If it has been sitting around, no oxidation and no reaction.

Geoff

Igor Nasonkin wrote:
> Hi everybody,
>
> I am looking for a detailed protocol for PAS staining in liver sections
> (paraffin-embedded). The 1st set of sections did not work, and we had no
> +control since we have not done it. These are 4-6wk mouse liver sections
> embedded in paraffin; our lab is experienced in IHC on paraffin sections so
> these were done right. But PAS staining did not work. What could be main
> reasons? Any positive control we could use? Thank you in advance,
> Igor
>
>
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>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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