[Histonet] RINSING IN TAP WATER AFTER DECAL

[Akemi Allison-Tacha]

Hi All,

I am going to put an additional tidbit into the mix. I was instructed many many moons ago, (back in 1974) to 1st rinse off any residual RDO or decal solution with a brief rinse in tap water and then place the decal specimens into saturated sodium bicarbonate for 10 minutes to stop the decal process, otherwise the specimen will continue to decal. The tissue fizzes a bit, when this reaction ceases, then rinse in running tap water for an additional 10 minutes. 

Akemi Allison-Tacha, BS, HT(ASCP)HTL
Client Services Manager
PhenoPath laboratories
551 North 34th Street, Suite 100 
Seattle, WA 98103-8675
Work: (206) 374-9000 ext 1053
E-Mail: akemiat3377 <@t> yahoo.com


--- On Tue, 5/6/08, Rena Fail <renafail <@t> bellsouth.net> wrote:

> From: Rena Fail <renafail <@t> bellsouth.net>
> Subject: RE: [Histonet] RINSING IN TAP WATER AFTER DECAL
> To: "'Fischer, R. B'" <BFicher <@t> chomp.org>, histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, May 6, 2008, 10:37 AM
> The rinse step is necessary between formalin and RDO, the
> two chemicals
> should not be combined
> Rena
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On
> Behalf Of Fischer,
> R. B
> Sent: Tuesday, May 06, 2008 12:22 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: Delcambre, Linda V
> Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL
> 
> We have been using "RDO" and "CAL-X"
> decal solutions for some time now.
> After the specified times in the decal solutions, the
> specimens are
> rinsed in running tap water for about 10 minutes before
> being placed
> back in 10%NBF for routine processing. We were rinsing to
> get rid of the
> nitric acid. My questions are (1) Is this rinse step
> necessary? If it
> is, (2) How long is an adequate time for rinsing?(3) How
> are other labs
> handling the specimen post decal, and before processing?
>  
>  
>  
> R.Brian Fischer
> Histology Lead Tech
> Community Hospital of the Monterey Peninsula
> PO Box HH Monterey Ca. 93942
> 831-625-4791
> Fax: 831-6583683
>  
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