[Histonet] IHC unclear at 40x

[Jo Dee Fish]

Bernard, 
Don't forget to do Koehler illumination procedure to your microscope as
well.  If you need instructions, try here:
http://www.rawlight.com/Koehler_illumination.pdf
Take care,
Jo Dee


~~Jo Dee Fish~~
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu

Mailing address:  
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 08, 2008 12:46 PM
To: Bernard Martin; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC unclear at 40x

Make sure that when you finish and dehydrate to coverslip, that dehydration
is complete before clearing the section with xylene.
  René J. 

Bernard Martin <bernardgerard <@t> yahoo.com> wrote:
  My IHC looks great at 5x, 10x, and 20x, but when I go up to 40x, it is a
bit blurry. The objective is clean, as is the slide itself. I deparaffinate
in clean xylene. Anyone run into this problem, and if so, how did you
resolve it? Thanks!


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