[Histonet] Her2 antibody

[ddittus787 <@t> aol.com]

As a side note zymed also has Tab 250 clone of cerb-2 that only need enzyme and then incubation and gives awesome reuslts as well.                           dana 
 
-----Original Message-----
From: ddittus787 <@t> aol.com
To: eca9 <@t> georgetown.edu; histonet <@t> lists.utsouthwestern.edu
Sent: Wed, 22 Jun 2005 16:29:03 -0400
Subject: Re: [Histonet] Her2 antibody


anti-her 2 cb11 clone works very well with citrate hier dilution(depending on 
your method is 1:40-1:100)
and a 30 minute incubation at 37 degrees.
                                                                        dana
 
 
 
 
 
 
 
 
 
 
 
 
 
-----Original Message-----
From: Eva C Andersson <eca9 <@t> georgetown.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, 22 Jun 2005 14:30:14 -0400
Subject: [Histonet] Her2 antibody


Hello, 
I just ordered mouse anti-Her2 cat.no. 18-7107 from Zymed. There is however no 
indication given as to which dilution to use. I want to use it for 
Immunohistochemistry on paraffin embedded sections. It also doesn't say anything 
about antigen retrieval. 
Is anyone using this antibody and if so could you tell me what you do? 
Thanks, 
Eva Andersson 
Georgetown University 
 
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