[Histonet] Her2 antibody

[ddittus787 <@t> aol.com]

anti-her 2 cb11 clone works very well with citrate hier dilution(depending on your method is 1:40-1:100)
and a 30 minute incubation at 37 degrees.
                                                                        dana
 
 
 
 
 
 
 
 
 
 
 
 
 
-----Original Message-----
From: Eva C Andersson <eca9 <@t> georgetown.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, 22 Jun 2005 14:30:14 -0400
Subject: [Histonet] Her2 antibody


Hello, 
I just ordered mouse anti-Her2 cat.no. 18-7107 from Zymed. There is however no indication given as to which dilution to use. I want to use it for Immunohistochemistry on paraffin embedded sections. It also doesn't say anything about antigen retrieval. 
Is anyone using this antibody and if so could you tell me what you do? 
Thanks, 
Eva Andersson 
Georgetown University 
 
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