[Histonet] RE: Histonet Digest, Vol 14, Issue 26
Dorothy.L.Webb <@t> HealthPartners.Com
Dorothy.L.Webb <@t> HealthPartners.Com
Fri Jan 21 10:26:11 CST 2005
We have been using the Mercedes coverslipping tape and find it adhering very
well. I switched this hospital over to it over a year ago due to the cost
savings and we have no trouble with lifting, even the older slides! There
are times that we have had a "splicing" withing the middle of the tape, but,
they replaced those and not for a long time has this happened. The tape is
slightly thicker, so you have to make certain your coverslipper is set to
push the tape down with the right amount of pressure.
Also, to the person cutting IHC's with the adhesive in it. We bought a
small waterbath to keep sterile water in and this is where the IHC's get
laid out on as well as out AFB's and Ahoramine Rhodamine stains. It has
worked well for us!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, January 21, 2005 9:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 14, Issue 26
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Today's Topics:
1. NIH image J surface area unit (Subratab)
2. Certification requirements (Fran Lemons)
3. frozens on fat to image GFP (Patsy Ruegg)
4. Rudeness on histonet (Randolph-Habecker, Julie)
5. Re: NIH image J surface area unit (Rocan)
6. Re: Certification requirements (Gareth Davis)
7. Re: Rudeness on histonet (Gareth Davis)
8. certification requirements to work in histo labs (Dawn Cowie)
9. Re: Rudeness on histonet (Robyn Vazquez)
10. HIPPA rules and fax transmittals going to wrong person
(Georgia Stewart)
11. RE: perfusion suggestions (Rittman, Barry)
12. Re: HIPPA rules and fax transmittals going to wrong person
(Victor Tobias)
13. RE: Rudeness on Histonet (mprice26 <@t> juno.com)
14. GLP lab staining question (pam plumlee)
15. Re: certification requirements to work in histo labs
(Gayle Callis)
16. Re: GLP lab staining question (Bryan Llewellyn)
17. Special stain (Linda Jones)
18. Re: Special stain (Jennifer MacDonald)
19. Bouins fixation problem (Kim O'Sullivan)
20. c-fos antibody in mouse tissue (Kim Merriam)
21. Coverslipping tape (Angela Bitting)
22. saturated fingertips (DPALLP <@t> aol.com)
23. Re: Bouins fixation problem (Geoff McAuliffe)
24. RE: Coverslipping tape (Rice, Michael)
25. RE: Rudeness on Histonet (Dawson, Glen)
26. Use of Sta-On for Immuno stains (KarBieber <@t> aol.com)
----------------------------------------------------------------------
Message: 1
Date: Fri, 21 Jan 2005 0:02:21 +0600
From: Subratab <subratab <@t> bdonline.com>
Subject: [Histonet] NIH image J surface area unit
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200501201804.j0KI4YYb008313 <@t> mailout.proshikanet.com>
Content-Type: text/plain; charset="iso-8859-1";
Dear Histo experts
My friend is measuring glomerular surface area by using Image J software. He
is in trouble to get the unit of the area calculated by the software, I
mean, if the calculated area is to be expressed in square milimeter or
square micrometer.
He is capturing the figures from microscope with X100 magnification
(oil-emersion lense) and then analyzing the glomerular surface area by the
software in a computer.
Could anybody please help to solve this.
Sincerely
Subrata Biswas
University of Campinas
SP, Brazil.
--
http://www.histosearch.com/homepages/DrSubrataKumarBiswas.html
------------------------------
Message: 2
Date: Thu, 20 Jan 2005 13:26:57 -0500
From: "Fran Lemons" <flemons <@t> bhset.org>
Subject: [Histonet] Certification requirements
To: <histonet <@t> lists.utsouthwestern.edu>,
<histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID: <s1efb1b2.095 <@t> bhsmail.baptistoneword.org>
Content-Type: text/plain; charset=US-ASCII
Has anyone out there seen anything in writing about not being able to work
in histology labs without HT certification?
Thanks
Fran Walker
------------------------------
Message: 3
Date: Thu, 20 Jan 2005 12:16:05 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] frozens on fat to image GFP
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200501201916.j0KJG02p023804 <@t> chip.viawest.net>
Content-Type: text/plain; charset="US-ASCII"
Help! Here is the situation. An investigator wants me to cut fat tissue
(briefly paraformaldehyde fixed,snap frozen in OCT) which does not cut well,
so I had the brillant (I thought) idea of using the Instrumedics tape
transfer system. I cut the sections and transfered them to the coated
slides, air dryed them, and they looked pretty good. Since they want to
preserve the fat I thought I would just use some aqueous mount and put a cs
on them so they could look at the sections under UV. Well there was some
kind of reaction from the ?water or glycerine or something that turned white
so we can't see the tissue. Tryed soaking the slides in water to remove
mount, the cs came off put the white reaction did not. so is the water
reacting with the polymer coating (?might be GMA). Should I take these
sections thru solvent anyway to get them coverslipped so they are clear???
Patsy
------------------------------
Message: 4
Date: Thu, 20 Jan 2005 11:27:09 -0800
From: "Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org>
Subject: [Histonet] Rudeness on histonet
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5E6BFDF4F0AB2C4DA69CF4473FC7B9484EAE7E <@t> wala01.seattlecca.org>
Content-Type: text/plain
Dear Kristen and others:
I too feel that Kristen was treated rudely. I have noticed this problem from
time to time on HistoNet and I feel I have to say something. Some people
generally assume that because you ask an open question that you are
clueless. While you might get better answers by being more specific, the
better way to respond to "any suggestions" is to ask if you could provide
more details. Just assuming that you and your boss have no idea what you are
doing and have ventured into this project naively is not fair. That response
was not helpful - it was nothing but rude. Did they suggest a class or a
book that they found practically good? No! The response was condescending
and pointless. Please ask for clarification before you humiliate a person.
And please do not tell someone they deserved to be treated rudely - that is
even worse!
People write into histonet with questions because there is a wealth of
knowledge amongst the subscribers. This is valuable information and
constitutes years and years of experience. Keep in mind, all of our
experiences have been different. Just because a person might not have
experience in one area doesn't mean that they are not very knowledgeable in
another. In other words, if you are rude to a person they might not want to
share valuable information with you when you ask. Or worse, they might leave
the Histonet and deprive all of us of their knowledge!
I too asked an open question one time about processing tissue with plastic
beads in it and received an incredibly condescending response instructing me
on how to design an experiment. I have a Ph.D. for god's sake. I have spent
15 years designing experiments. I also saw a young student publicly filleted
for cheating when her instructor encouraged her to send her inquiry out to
the Histonet.
Kristen, please don't leave the histonet because of one (incredibly) rude
response. Take it for what it is - useless! Please hold out for helpful
people that actually have information to contribute.
Hang in there!
Julie
Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org <mailto:jhabecke <@t> fhcrc.org>
Message: 2
Date: Thu, 20 Jan 2005 11:44:27 -0800
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] perfusion suggestions follow up
To: Kristen Reynolds <kristenhinkle <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <41F00A1B.6060101 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Dear Kristen:
Your original posting (see below) gave no indication that you were
familiar with perfusion for LM or EM. You did not ask for fixative
composition, you just asked for "any suggestions". Given the broad scope
of your question, the advice you got WAS helpful. The fixative
combination for wanting to do both on the same tissue IS out there, but
you have to be more specific in your question. The correct fixative
combinatio will depend on:
1. Are you doing histochemistry? If so, what reaction? Pre-embedding or
post-embedding reaction?
2. Are you doing immunohistochemistry? If so, what antigen?
Pre-embedding or post-embedding reaction?
3. What plastic are you embedding in?
4. What routine stains do you need to do?
5. What part of the brain will you be looking at? The brain is a big place.
6. Do you need to do LM and EM on the same piece of tissue/same cells?
Advice on HistoNet is free. Some of it is quite good. Checking published
papers in refereed journals and looking at the results is always a good
idea, espcially given the cost of experimental animals.
Geoff
Kristen Reynolds wrote:
I need advice on perfusion solutions for rat/monkey
brains. I need to use the tissue for both light
microscopy and EM. Any suggestions?
>Wow, I can't believe how rude of a response I got. I
>thought this was for helpful comments. I know how to
>perfuse for light microscopy and EM. I just thought
>the fixative combination for wanting to do both on the
>same tissue might be out there. I guess I won't be
>asking histonet anything anymore.
>
>__________________________________________________
>
This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying, distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.
------------------------------
Message: 5
Date: Thu, 20 Jan 2005 11:28:47 -0800
From: Rocan <rocan <@t> mac.com>
Subject: Re: [Histonet] NIH image J surface area unit
To: "'histonet <@t> pathology.swmed.edu'" <histonet <@t> pathology.swmed.edu>,
Subratab <subratab <@t> bdonline.com>
Message-ID: <816F081F-6B19-11D9-8D0A-000A9589219E <@t> mac.com>
Content-Type: text/plain; charset=US-ASCII; format=flowed
I guess there are a couple of ways to do this. I use a stage micrometer
(you can google "stage micrometer" and find it on the pictures). I
take a picture of the ruler imprinted. Typically these rulers have a
one millimeter ruler and 100 divisions of 10 micrometers each. So,
with the photo using the same scope and objective you go to any of
these programs like NIH ImageJ and open the document (same size as the
photos you are measuring). There is a command where you place the mouse
on one end drag and click on the other end of the markings. So, if you
drag it over ten marking you then instruct the program that that
distance is 100 micrometers. Then using these settings you do your
measurements and the measurements would then be very precise and will
be in micrometers. I do this with a different program but I know you
can do it with ImageJ.
I hope this helps.
Rocio
-----
Dr.Rocio Sierra-Honigmann
Director
Engineered Wound Repair Laboratory
Cedars Sinai Research Institute
Davis 1091
310-423-1882
Honigmannr <@t> cshs.org
On Jan 20, 2005, at 10:02 AM, Subratab wrote:
> Dear Histo experts
> My friend is measuring glomerular surface area by using Image J
> software. He
> is in trouble to get the unit of the area calculated by the software, I
> mean, if the calculated area is to be expressed in square milimeter or
> square micrometer.
> He is capturing the figures from microscope with X100 magnification
> (oil-emersion lense) and then analyzing the glomerular surface area by
> the
> software in a computer.
> Could anybody please help to solve this.
> Sincerely
>
> Subrata Biswas
> University of Campinas
> SP, Brazil.
>
> --
> http://www.histosearch.com/homepages/DrSubrataKumarBiswas.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Thu, 20 Jan 2005 12:09:42 -0800 (PST)
From: Gareth Davis <mrsgbd2001 <@t> yahoo.com>
Subject: Re: [Histonet] Certification requirements
To: Fran Lemons <flemons <@t> bhset.org>, Histonet
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050120200942.71181.qmail <@t> web52708.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Fran,
I believe it's based on what state you work in. I know that Florida
requires an ASCP certification as well as a Florida license.
Tennessee, where I work, does not require it.
Gareth Davis
Fran Lemons <flemons <@t> bhset.org> wrote:
Has anyone out there seen anything in writing about not being able to work
in histology labs without HT certification?
Thanks
Fran Walker
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Do you Yahoo!?
Yahoo! Search presents - Jib Jab's 'Second Term'
------------------------------
Message: 7
Date: Thu, 20 Jan 2005 12:14:32 -0800 (PST)
From: Gareth Davis <mrsgbd2001 <@t> yahoo.com>
Subject: Re: [Histonet] Rudeness on histonet
To: "Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org>, Histonet
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050120201432.90980.qmail <@t> web52710.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Here! Here! to Julie.
I have also experienced the rudeness and seen others put down and treated as
if they were stupid. It's very discouraging.
Gareth
"Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org> wrote:
Dear Kristen and others:
I too feel that Kristen was treated rudely. I have noticed this problem from
time to time on HistoNet and I feel I have to say something. Some people
generally assume that because you ask an open question that you are
clueless. While you might get better answers by being more specific, the
better way to respond to "any suggestions" is to ask if you could provide
more details. Just assuming that you and your boss have no idea what you are
doing and have ventured into this project naively is not fair. That response
was not helpful - it was nothing but rude. Did they suggest a class or a
book that they found practically good? No! The response was condescending
and pointless. Please ask for clarification before you humiliate a person.
And please do not tell someone they deserved to be treated rudely - that is
even worse!
People write into histonet with questions because there is a wealth of
knowledge amongst the subscribers. This is valuable information and
constitutes years and years of experience. Keep in mind, all of our
experiences have been different. Just because a person might not have
experience in one area doesn't mean that they are not very knowledgeable in
another. In other words, if you are rude to a person they might not want to
share valuable information with you when you ask. Or worse, they might leave
the Histonet and deprive all of us of their knowledge!
I too asked an open question one time about processing tissue with plastic
beads in it and received an incredibly condescending response instructing me
on how to design an experiment. I have a Ph.D. for god's sake. I have spent
15 years designing experiments. I also saw a young student publicly filleted
for cheating when her instructor encouraged her to send her inquiry out to
the Histonet.
Kristen, please don't leave the histonet because of one (incredibly) rude
response. Take it for what it is - useless! Please hold out for helpful
people that actually have information to contribute.
Hang in there!
Julie
Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org
Message: 2
Date: Thu, 20 Jan 2005 11:44:27 -0800
From: Geoff McAuliffe
Subject: Re: [Histonet] perfusion suggestions follow up
To: Kristen Reynolds
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <41F00A1B.6060101 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Dear Kristen:
Your original posting (see below) gave no indication that you were
familiar with perfusion for LM or EM. You did not ask for fixative
composition, you just asked for "any suggestions". Given the broad scope
of your question, the advice you got WAS helpful. The fixative
combination for wanting to do both on the same tissue IS out there, but
you have to be more specific in your question. The correct fixative
combinatio will depend on:
1. Are you doing histochemistry? If so, what reaction? Pre-embedding or
post-embedding reaction?
2. Are you doing immunohistochemistry? If so, what antigen?
Pre-embedding or post-embedding reaction?
3. What plastic are you embedding in?
4. What routine stains do you need to do?
5. What part of the brain will you be looking at? The brain is a big place.
6. Do you need to do LM and EM on the same piece of tissue/same cells?
Advice on HistoNet is free. Some of it is quite good. Checking published
papers in refereed journals and looking at the results is always a good
idea, espcially given the cost of experimental animals.
Geoff
Kristen Reynolds wrote:
I need advice on perfusion solutions for rat/monkey
brains. I need to use the tissue for both light
microscopy and EM. Any suggestions?
>Wow, I can't believe how rude of a response I got. I
>thought this was for helpful comments. I know how to
>perfuse for light microscopy and EM. I just thought
>the fixative combination for wanting to do both on the
>same tissue might be out there. I guess I won't be
>asking histonet anything anymore.
>
>__________________________________________________
>
This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying, distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Do you Yahoo!?
Yahoo! Search presents - Jib Jab's 'Second Term'
------------------------------
Message: 8
Date: Thu, 20 Jan 2005 12:49:37 -0800 (PST)
From: Dawn Cowie <dlcowie <@t> prodigy.net>
Subject: [Histonet] certification requirements to work in histo labs
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050120204937.7153.qmail <@t> web81008.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Fran,
In Florida where I am, to perform tech duties you must be licensed. However,
you may employ non techs to work as lab aides. These aides may perform a
wide variety of duties that usually are done by techs. Specifically what
they cannot do is: embed, cut, stain, frozen sections etc. What they can do
is: change reagents, coverslip, start and stop an automated stainer. I hope
this helps.
Dawn Cowie, HT
Pensacola Path
------------------------------
Message: 9
Date: Thu, 20 Jan 2005 12:57:54 -0800
From: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>
Subject: Re: [Histonet] Rudeness on histonet
To: histonet <@t> lists.utsouthwestern.edu, jhabecke <@t> seattlecca.org
Message-ID: <s1efaadd.026 <@t> gwsmtp.ohsu.edu>
Content-Type: text/plain; charset=us-ascii
BRAVO ladies for speaking your mind!!!! :0)
Robyn
>>> "Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org> 1/20/2005
11:27:09 AM >>>
Dear Kristen and others:
I too feel that Kristen was treated rudely. I have noticed this problem
from
time to time on HistoNet and I feel I have to say something. Some
people
generally assume that because you ask an open question that you are
clueless. While you might get better answers by being more specific,
the
better way to respond to "any suggestions" is to ask if you could
provide
more details. Just assuming that you and your boss have no idea what
you are
doing and have ventured into this project naively is not fair. That
response
was not helpful - it was nothing but rude. Did they suggest a class or
a
book that they found practically good? No! The response was
condescending
and pointless. Please ask for clarification before you humiliate a
person.
And please do not tell someone they deserved to be treated rudely -
that is
even worse!
People write into histonet with questions because there is a wealth of
knowledge amongst the subscribers. This is valuable information and
constitutes years and years of experience. Keep in mind, all of our
experiences have been different. Just because a person might not have
experience in one area doesn't mean that they are not very
knowledgeable in
another. In other words, if you are rude to a person they might not
want to
share valuable information with you when you ask. Or worse, they might
leave
the Histonet and deprive all of us of their knowledge!
I too asked an open question one time about processing tissue with
plastic
beads in it and received an incredibly condescending response
instructing me
on how to design an experiment. I have a Ph.D. for god's sake. I have
spent
15 years designing experiments. I also saw a young student publicly
filleted
for cheating when her instructor encouraged her to send her inquiry out
to
the Histonet.
Kristen, please don't leave the histonet because of one (incredibly)
rude
response. Take it for what it is - useless! Please hold out for
helpful
people that actually have information to contribute.
Hang in there!
Julie
Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org <mailto:jhabecke <@t> fhcrc.org>
Message: 2
Date: Thu, 20 Jan 2005 11:44:27 -0800
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] perfusion suggestions follow up
To: Kristen Reynolds <kristenhinkle <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <41F00A1B.6060101 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Dear Kristen:
Your original posting (see below) gave no indication that you were
familiar with perfusion for LM or EM. You did not ask for fixative
composition, you just asked for "any suggestions". Given the broad
scope
of your question, the advice you got WAS helpful. The fixative
combination for wanting to do both on the same tissue IS out there, but
you have to be more specific in your question. The correct fixative
combinatio will depend on:
1. Are you doing histochemistry? If so, what reaction? Pre-embedding or
post-embedding reaction?
2. Are you doing immunohistochemistry? If so, what antigen?
Pre-embedding or post-embedding reaction?
3. What plastic are you embedding in?
4. What routine stains do you need to do?
5. What part of the brain will you be looking at? The brain is a big
place.
6. Do you need to do LM and EM on the same piece of tissue/same cells?
Advice on HistoNet is free. Some of it is quite good. Checking
published
papers in refereed journals and looking at the results is always a good
idea, espcially given the cost of experimental animals.
Geoff
Kristen Reynolds wrote:
I need advice on perfusion solutions for rat/monkey
brains. I need to use the tissue for both light
microscopy and EM. Any suggestions?
>Wow, I can't believe how rude of a response I got. I
>thought this was for helpful comments. I know how to
>perfuse for light microscopy and EM. I just thought
>the fixative combination for wanting to do both on the
>same tissue might be out there. I guess I won't be
>asking histonet anything anymore.
>
>__________________________________________________
>
This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying,
distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 20 Jan 2005 12:58:14 -0800 (PST)
From: Georgia Stewart <stewart_georgia <@t> sbcglobal.net>
Subject: [Histonet] HIPPA rules and fax transmittals going to wrong
person
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050120205814.70166.qmail <@t> web81402.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello and Good Afternoon Everyone
In this era of HIPPA rules and regulations in the United States, I have not
seen any comment on HIPPA as it relates to fax transmittal's of reports that
might go to the wrong person/facility. If a report goes to the the wrong
person/facility, isn't this is a breach of patient confidentiality covered
by HIPPA? How are you handling faxs that go, or might go, to the wrong
person/facility?
Appreciate your comments.
Georgia Stewart, BS, HTL
Georgia
------------------------------
Message: 11
Date: Thu, 20 Jan 2005 15:00:39 -0600
From: "Rittman, Barry" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] perfusion suggestions
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<566FB0B522443D43AF02D2ADBE35A6F0012C92C3 <@t> UTHEVS3.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Kristen
I am not trying to justify any response that you received but I think
that I understand why the response was phrased in that particular
manner.
Many of the questions that arrive at Histonet are very specific, and
generally get a lot of responses.
The broader questions are often difficult to answer (and in general I
personally do not respond to these) and often receive few responses.
It is usually not possible to determine the level of knowledge of the
individual who is asking the question and there may be a wide variety of
answers.
Here on Histonet we are often between a rock and a hard place. I believe
that it is a natural tendency nowadays to pose the question in the
shortest way possible on the assumption that nobody wishes to spend
their time to read a long discourse. This unfortunately often results in
a communication that is open to a wide variety of interpretations. One
interpretation is that the individual posing the question wants to be
informed about all aspects of a particular technique from anesthetizing
an animal to interpreting the final result, rather than carrying out the
initial groundwork themselves. There is usually no way to judge if this
is true from the original communications.
This is the era of rapid fire information but unfortunately the
recipients do not always have the same background or experiences to view
that information in the same light.
In many cases I feel that in response to complex questions, individuals
with the appropriate expertise should provide their telephone number so
that a dialogue can be set up. While Histonet is very useful, a one on
one dialogue generally will provide the most relevant information.
I hope that you will continue to ask questions on Histonet.
Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristen
Reynolds
Sent: Thursday, January 20, 2005 9:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] perfusion suggestions
Wow, I can't believe how rude of a response I got. I
thought this was for helpful comments. I know how to
perfuse for light microscopy and EM. I just thought
the fixative combination for wanting to do both on the
same tissue might be out there. I guess I won't be
asking histonet anything anymore.
__________________________________________________
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------------------------------
Message: 12
Date: Thu, 20 Jan 2005 13:24:08 -0800
From: Victor Tobias <victor <@t> pathology.washington.edu>
Subject: Re: [Histonet] HIPPA rules and fax transmittals going to
wrong person
To: Georgia Stewart <stewart_georgia <@t> sbcglobal.net>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <41F02178.1080606 <@t> pathology.washington.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
We had an incident where a patient report went to a Barnes and Noble
bookstore. At the time of the incident many users had access to our
database for inputting new referring physicians. It is very easy to make
a typo and the fax is going who knows where. The bookstore contacted us
regarding the incident. We confirmed that we had their fax number in our
database. An incident report was filed with risk management. Since the
incident, we have restricted access to our database. Our facility
recommendation is to verify fax numbers quarterly. With our staffing
levels this is not feasible. We do send out annual verifications, that
whoever signs the form is stating the fax number is correct and the
machine is in a secure location. We will make several attempts to get
the verification back. If there is no response then we will stop faxing
to them and send a print copy through the mail. We have custom reports
created to query the database when a physician is listed on a current
case and their fax verification is more than a year old. Our facility
policy states that a reasonable effort will be made to verify a fax
number. What happens when a doctors office calls and asks for a copy of
a patient report? We document in the database who we sent a copy of the
report to.
Victor
Georgia Stewart wrote:
>Hello and Good Afternoon Everyone
>
>In this era of HIPPA rules and regulations in the United States, I have not
seen any comment on HIPPA as it relates to fax transmittal's of reports that
might go to the wrong person/facility. If a report goes to the the wrong
person/facility, isn't this is a breach of patient confidentiality covered
by HIPPA? How are you handling faxs that go, or might go, to the wrong
person/facility?
>
>Appreciate your comments.
>
>Georgia Stewart, BS, HTL
>
>
>
>Georgia
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
--
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
=================================================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.
------------------------------
Message: 13
Date: Thu, 20 Jan 2005 22:04:33 GMT
From: "mprice26 <@t> juno.com" <mprice26 <@t> juno.com>
Subject: [Histonet] RE: Rudeness on Histonet
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050120.140511.23783.21286 <@t> webmail26.nyc.untd.com>
Content-Type: text/plain
I have been treated rudely also. I have e-mailed the histonet for a vendor's
# or for other info and I will have people rerspond back with try google
search.
I know how to search for something via google or yellowpages.com but prefer
to use the histonet because of the vast number of vendors that subscribe to
the histonet. So not only do I receive the # I am asking for I also receive
info from other vendors that I would not receive if I look it up on google.
I wish those that think I am an idiot for not using google would just not
respond to my request. If it bothers them so much that I am requesting info
via the Histonet. It takes much less time to hit their delete button than it
does for them to e-mail me a rude, condescending message.
I would like to add that for the most part I receive prompt useful replys,
it is just a few people that reply with rude answers.
Marsha Price
------------------------------
Message: 14
Date: Thu, 20 Jan 2005 14:13:17 -0800 (PST)
From: pam plumlee <paw555 <@t> yahoo.com>
Subject: [Histonet] GLP lab staining question
To: HistoNet Server <histonet <@t> pathology.swmed.edu>
Message-ID: <20050120221317.48866.qmail <@t> web11605.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello fellow histonetters: I'm setting up a special
stain control/slide bank for our research pharm lab.
I'm curious how special stain controls are validated.
Is it as simple as screening the stained slides/blocks
as they are cut and determining if they are positive
or not, along with the proper documentation? Anyone
care to share info? Thanks, PP
__________________________________
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------------------------------
Message: 15
Date: Thu, 20 Jan 2005 15:14:25 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] certification requirements to work in histo
labs
To: Dawn Cowie <dlcowie <@t> prodigy.net>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050120150521.01b1fbc0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I am curious or rather need clarification here. Licensed means licensed by
the state but HT(SCP) is a certification granted by a certification agency,
ASCP/CAP. I don't think ASCP/CAP is governed by any state's' licensing
regulations.
Many moons ago, in New York, one did not have to be certified as an HT to
be licensed by the state (one had to take a test for NY licensure), and
there were NY licensed technicians without HT(ASCP) certification
performing tech duties and, in fact, was the supervisor of our
histopathology lab.
At 01:49 PM 1/20/2005, you wrote:
>Fran,
>
>In Florida where I am, to perform tech duties you must be licensed.
>However, you may employ non techs to work as lab aides. These aides may
>perform a wide variety of duties that usually are done by techs.
>Specifically what they cannot do is: embed, cut, stain, frozen sections
>etc. What they can do is: change reagents, coverslip, start and stop an
>automated stainer. I hope this helps.
>
>Dawn Cowie, HT
>
>Pensacola Path
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 16
Date: Thu, 20 Jan 2005 14:36:24 -0800
From: Bryan Llewellyn <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] GLP lab staining question
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001601c4ff40$7923b6b0$80004246 <@t> yourlk4rlmsu>
Content-Type: text/plain; reply-type=original; charset=iso-8859-1;
format=flowed
The way I was taught was to cut a series of sections, 50 or so, then stain
the first and the last to make sure they were positive If they both are, it
is presumed that all the others are also. If not, stain intermediate
sections until one is positive, then use from the first one to that.
Bryan Llewellyn
----- Original Message -----
From: "pam plumlee" <paw555 <@t> yahoo.com>
To: "HistoNet Server" <histonet <@t> pathology.swmed.edu>
Sent: Thursday, January 20, 2005 2:13 PM
Subject: [Histonet] GLP lab staining question
> Hello fellow histonetters: I'm setting up a special
> stain control/slide bank for our research pharm lab.
> I'm curious how special stain controls are validated.
> Is it as simple as screening the stained slides/blocks
> as they are cut and determining if they are positive
> or not, along with the proper documentation? Anyone
> care to share info? Thanks, PP
>
>
>
> __________________________________
> Do you Yahoo!?
> Take Yahoo! Mail with you! Get it on your mobile phone.
> http://mobile.yahoo.com/maildemo
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Thu, 20 Jan 2005 16:45:10 -0600
From: "Linda Jones" <ljones <@t> pathology.umsmed.edu>
Subject: [Histonet] Special stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s1efe01d.082 <@t> GWIA1.umsmed.edu>
Content-Type: text/plain; charset=US-ASCII
How do you charge for certain stains? Should charge due to the length of
time it take to do the stain or the price of the chemicals?
thanks
Linda Harper-Jones BS.,HT/ HTL(ASCP)
University of Mississippi Medical Center
Department of Pathology
Chief Histotechnologist Supervisor
(601) 984-1576
(601) 984-4968 Fax
ljones <@t> pathology.umsmed.edu
------------------------------
Message: 18
Date: Thu, 20 Jan 2005 15:49:45 -0800
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Special stain
To: "Linda Jones" <ljones <@t> pathology.umsmed.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF0D388D8F.CA46DB2D-ON88256F8F.0082BFE2-88256F8F.0083B446 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
Special stains are based on the type of stain. For billing purposes
there are two types: microorganism or not. 88312 and 88313. Cost or
length of time is not a factor when billing.
"Linda Jones" <ljones <@t> pathology.umsmed.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/20/2005 02:45 PM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Special stain
How do you charge for certain stains? Should charge due to the length of
time it take to do the stain or the price of the chemicals?
thanks
Linda Harper-Jones BS.,HT/ HTL(ASCP)
University of Mississippi Medical Center
Department of Pathology
Chief Histotechnologist Supervisor
(601) 984-1576
(601) 984-4968 Fax
ljones <@t> pathology.umsmed.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Fri, 21 Jan 2005 04:04:26 +0000
From: Kim O'Sullivan <Kim.Osullivan <@t> med.monash.edu.au>
Subject: [Histonet] Bouins fixation problem
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <130.194.114.210.1106279796.85555 <@t> my.monash.edu.au>
Content-Type: text/plain
Hi everybody,
We have used bouin's fixative for fixing mouse kidneys for years with no
problem, and have recently been experiencing problems cutting them once they
have been processed and wax embedded. The kidney tissue has holes in it in
the medulla region, is very difficult to cut(tissue brittle) and it appears
almost like the wax has not infiltrated the kidneys properly. Additonally
once the sections have been mounted on glass slides they appear fine but
when we go to PAS stain them half of them come off the slides in solution
(this has never happened before in the last 3 years)I have checked the
processing schedule and that is fine (as is all the solutions) I have
checked the temperature of the wax (which is fine). Does anyone out there
know what our problem may be?? Additionally is there any way that any of the
solutions used to make bouin's (picric acid, formalin and glacial acetic
acid)can go off? And how would you check for this.
Would appreciate any help!!
Kim O'Sullivan
------------------------------
Message: 20
Date: Fri, 21 Jan 2005 05:24:15 -0800 (PST)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] c-fos antibody in mouse tissue
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050121132416.20426.qmail <@t> web52502.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello All,
I was wondering if anyone knew what tissue would make the best positive
control for this antibody in mouse tissue.
Kim Merriam
Novartis
Cambridge, MA
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------------------------------
Message: 21
Date: Fri, 21 Jan 2005 09:23:30 -0500
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] Coverslipping tape
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s1f0ca25.034 <@t> GHSGWIANW2.GEISINGER.EDU>
Content-Type: text/plain; charset=US-ASCII
What is the concensus of opinion on Mercedes Medicals coverslipping
tape?
I got a sample to try and the girls I work with said they had it before,
(I don't know how long ago) and in a couple days it started lifting.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
------------------------------
Message: 22
Date: Fri, 21 Jan 2005 09:25:41 EST
From: DPALLP <@t> aol.com
Subject: [Histonet] saturated fingertips
To: histonet <@t> pathology.swmed.edu
Message-ID: <191.372a8459.2f226ae5 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Does anyone have any suggestions to alleviate water saturated fingertips
from handling blocks from ice trays? Wearing gloves is too cumbersome and
the
different barrier lotions that we have tried do not seem to have an effect.
Susie
------------------------------
Message: 23
Date: Fri, 21 Jan 2005 09:39:29 -0800
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Bouins fixation problem
To: Kim O'Sullivan <Kim.Osullivan <@t> med.monash.edu.au>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <41F13E51.1010507 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Hi Kim:
Hmm. Certainly sounds like an infiltration problem. My first though
is that you are not getting all of the alcohol out of the medulla so
xylene (or substitute) is not getting in so the wax is not getting in.
When you say that you have checked all of the processing solutions and
they are fine, how did you check them? What is the criteria for deciding
that a solution is good? I would dump ALL of the processing solutions
and ALL of the wax and start fresh. Run some kidneys, cut them, and see
if that solves the problem. There is a small chance that the
manufacturer of your wax has changed the formula but with modern waxes
and additives such a change seems unlikely to cause problems.
As for reagents going bad, picric acid last forever, formaldehyde is
fine as long as there is no ppt. on the bottom of the bottle, I don't
think glacial acetic acid goes bad. Even if the fixation was incomplete
(you did not say how long you fix the kidneys, if you perfuse fixative,
or if you slice them open) the alcohols should finish the fixation.
Good luck!
Geoff
Kim O'Sullivan wrote:
>Hi everybody,
>
>We have used bouin's fixative for fixing mouse kidneys for years with no
problem, and have recently been experiencing problems cutting them once they
have been processed and wax embedded. The kidney tissue has holes in it in
the medulla region, is very difficult to cut(tissue brittle) and it appears
almost like the wax has not infiltrated the kidneys properly. Additonally
once the sections have been mounted on glass slides they appear fine but
when we go to PAS stain them half of them come off the slides in solution
(this has never happened before in the last 3 years)I have checked the
processing schedule and that is fine (as is all the solutions) I have
checked the temperature of the wax (which is fine). Does anyone out there
know what our problem may be?? Additionally is there any way that any of the
solutions used to make bouin's (picric acid, formalin and glacial acetic
acid)can go off? And how would you check for this.
>
>Would appreciate any help!!
>
>Kim O'Sullivan
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 24
Date: Fri, 21 Jan 2005 10:00:37 -0500
From: "Rice, Michael" <Michael.Rice <@t> holy-cross.com>
Subject: RE: [Histonet] Coverslipping tape
To: "Angela Bitting" <akbitting <@t> geisinger.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3BC92F29BE821745AB15E04C98EE028D693742 <@t> HCH2KMAIL.holy-cross.com>
Content-Type: text/plain
I have been using it for several months now and have seen no evidence of
lifting, not to say that it wont happen in the future. A friend of mine has
beeen using it for several years now with no problems
Mike Rice
Holy Cross hospital
ft lauderdale
-----Original Message-----
From: Angela Bitting [mailto:akbitting <@t> geisinger.edu]
Sent: Friday, January 21, 2005 9:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Coverslipping tape
What is the concensus of opinion on Mercedes Medicals coverslipping
tape?
I got a sample to try and the girls I work with said they had it before,
(I don't know how long ago) and in a couple days it started lifting.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
----------------------
Confidentiality Notice: Prepared in Anticipation of Litigation.
Attorney-Client Privileged. This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
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------------------------------
Message: 25
Date: Fri, 21 Jan 2005 09:01:05 -0600
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: [Histonet] RE: Rudeness on Histonet
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <D2401DE71F59D71184BC00D0B7479B91A58D93 <@t> MILW_MAIL1>
Content-Type: text/plain; charset="iso-8859-1"
Rude people on a listserver like this are unavoidable. Unfortunately, there
is no "pre-membership rudeness testing". If you are tired of being chewed
on like a bloody wildabeast in the midst of a den of lions, do what I was
forced to do and avoid lengthy general postings as much as possible. Submit
a pithy comment/question and pursue only those who seem to genuinely want to
help and delete those who are jerks. Trying to rail against rudeness can
become a full time job which doesn't pay anything.
Histonet is too valuable a tool to just get rid of, though, at times, it is
tempting.
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
------------------------------
Message: 26
Date: Fri, 21 Jan 2005 10:10:31 EST
From: KarBieber <@t> aol.com
Subject: [Histonet] Use of Sta-On for Immuno stains
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <194.36bbc139.2f227567 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
I was just wondering how other labs are handling this situation: For
prostate and breast core biopsies we cut extra slides for potential immuno
staining,
between the levels. The problem is the use of adhesive in the water bath.
Since we are cutting H&E's, there is the possibility of fall off during
staining
if we DON'T use sta-on. But using it increases the possibility of fall off
during immunos staining. And if we don't use the sta-on, we run the risk of
fall off on ALL the cases the techs are cutting that day.
I know there are lots of labs out there performing immunos, so I'd like to
know how you've resolved this.
Thanks,
Karen
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 14, Issue 26
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