[Histonet] RE: Histonet Digest, Vol 14, Issue 26

[Miller, Ann-Marie]

Karen,

We save intervening levels between our prostates and breasts as well.  One
tech will cut those first with just distilled water on positively charged
slides.  Then he'll add gelatin to the water and cut everything else.  Hope
this helps.

Ann

-----Original Message-----
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[mailto:histonet-request <@t> lists.utsouthwestern.edu]
Sent: None
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 14, Issue 26


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Today's Topics:

   1. NIH image J surface area unit (Subratab)
   2. Certification requirements (Fran Lemons)
   3. frozens on fat to image GFP (Patsy Ruegg)
   4. Rudeness on histonet (Randolph-Habecker, Julie)
   5. Re: NIH image J surface area unit (Rocan)
   6. Re: Certification requirements (Gareth Davis)
   7. Re: Rudeness on histonet (Gareth Davis)
   8. certification requirements to work in histo labs (Dawn Cowie)
   9. Re: Rudeness on histonet (Robyn Vazquez)
  10. HIPPA rules and fax transmittals going to wrong person
      (Georgia Stewart)
  11. RE: perfusion suggestions (Rittman, Barry)
  12. Re: HIPPA rules and fax transmittals going to wrong person
      (Victor Tobias)
  13. RE: Rudeness on Histonet (mprice26 <@t> juno.com)
  14. GLP lab staining question (pam plumlee)
  15. Re: certification requirements to work in histo labs
      (Gayle Callis)
  16. Re: GLP lab staining question (Bryan Llewellyn)
  17. Special stain (Linda  Jones)
  18. Re: Special stain (Jennifer MacDonald)
  19. Bouins fixation problem (Kim O'Sullivan)
  20. c-fos antibody in mouse tissue (Kim Merriam)
  21. Coverslipping tape (Angela Bitting)
  22. saturated fingertips (DPALLP <@t> aol.com)
  23. Re: Bouins fixation problem (Geoff McAuliffe)
  24. RE: Coverslipping tape (Rice, Michael)
  25. RE: Rudeness on Histonet (Dawson, Glen)
  26. Use of Sta-On for Immuno stains (KarBieber <@t> aol.com)


----------------------------------------------------------------------

Message: 1
Date: Fri, 21 Jan 2005 0:02:21 +0600
From: Subratab <subratab <@t> bdonline.com>
Subject: [Histonet] NIH image J surface area unit
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200501201804.j0KI4YYb008313 <@t> mailout.proshikanet.com>
Content-Type: text/plain; charset="iso-8859-1";

Dear Histo experts
My friend is measuring glomerular surface area by using Image J software. He
is in trouble to get the unit of the area calculated by the software, I
mean, if the calculated area is to be expressed in square milimeter or
square micrometer.
He is capturing the figures from microscope with X100 magnification
(oil-emersion lense) and then analyzing the glomerular surface area by the
software in a computer.
Could anybody please help to solve this.
Sincerely

Subrata Biswas
University of Campinas
SP, Brazil.

--
http://www.histosearch.com/homepages/DrSubrataKumarBiswas.html




------------------------------

Message: 2
Date: Thu, 20 Jan 2005 13:26:57 -0500
From: "Fran Lemons" <flemons <@t> bhset.org>
Subject: [Histonet] Certification requirements
To: <histonet <@t> lists.utsouthwestern.edu>,
	<histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID: <s1efb1b2.095 <@t> bhsmail.baptistoneword.org>
Content-Type: text/plain; charset=US-ASCII

Has anyone out there seen anything in writing about not being able to work
in histology labs without HT certification?
Thanks
Fran Walker




------------------------------

Message: 3
Date: Thu, 20 Jan 2005 12:16:05 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] frozens on fat to image GFP
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200501201916.j0KJG02p023804 <@t> chip.viawest.net>
Content-Type: text/plain;	charset="US-ASCII"

Help!  Here is the situation.  An investigator wants me to cut fat tissue
(briefly paraformaldehyde fixed,snap frozen in OCT) which does not cut well,
so I had the brillant (I thought) idea of using the Instrumedics tape
transfer system.  I cut the sections and transfered them to the coated
slides, air dryed them, and they looked pretty good.  Since they want to
preserve the fat I thought I would just use some aqueous mount and put a cs
on them so they could look at the sections under UV.  Well there was some
kind of reaction from the ?water or glycerine or something that turned white
so we can't see the tissue.  Tryed soaking the slides in water to remove
mount, the cs came off put the white reaction did not.  so is the water
reacting with the polymer coating (?might be GMA).  Should I take these
sections thru solvent anyway to get them coverslipped so they are clear???
Patsy


------------------------------

Message: 4
Date: Thu, 20 Jan 2005 11:27:09 -0800
From: "Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org>
Subject: [Histonet] Rudeness on histonet
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5E6BFDF4F0AB2C4DA69CF4473FC7B9484EAE7E <@t> wala01.seattlecca.org>
Content-Type: text/plain

Dear Kristen and others:

I too feel that Kristen was treated rudely. I have noticed this problem from
time to time on HistoNet and I feel I have to say something. Some people
generally assume that because you ask an open question that you are
clueless. While you might get better answers by being more specific, the
better way to respond to "any suggestions" is to ask if you could provide
more details. Just assuming that you and your boss have no idea what you are
doing and have ventured into this project naively is not fair. That response
was not helpful - it was nothing but rude. Did they suggest a class or a
book that they found practically good? No! The response was condescending
and pointless. Please ask for clarification before you humiliate a person.
And please do not tell someone they deserved to be treated rudely - that is
even worse!

People write into histonet with questions because there is a wealth of
knowledge amongst the subscribers. This is valuable information and
constitutes years and years of experience. Keep in mind, all of our
experiences have been different. Just because a person might not have
experience in one area doesn't mean that they are not very knowledgeable in
another. In other words, if you are rude to a person they might not want to
share valuable information with you when you ask. Or worse, they might leave
the Histonet and deprive all of us of their knowledge! 

I too asked an open question one time about processing tissue with plastic
beads in it and received an incredibly condescending response instructing me
on how to design an experiment. I have a Ph.D. for god's sake. I have spent
15 years designing experiments. I also saw a young student publicly filleted
for cheating when her instructor encouraged her to send her inquiry out to
the Histonet. 

Kristen, please don't leave the histonet because of one (incredibly) rude
response. Take it for what it is - useless! Please hold out for helpful
people that actually have information to contribute.

Hang in there!

Julie

Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org <mailto:jhabecke <@t> fhcrc.org>




Message: 2
Date: Thu, 20 Jan 2005 11:44:27 -0800
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] perfusion suggestions follow up
To: Kristen Reynolds <kristenhinkle <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <41F00A1B.6060101 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1

Dear Kristen:

    Your original posting (see below) gave no indication that you were 
familiar with perfusion for LM or EM. You did not ask for fixative 
composition, you just asked for "any suggestions". Given the broad scope 
of your question, the advice you got WAS helpful. The fixative 
combination for wanting to do both on the same tissue IS out there, but 
you have to be more specific in your question. The correct fixative 
combinatio will depend on:
1. Are you doing histochemistry? If so, what reaction? Pre-embedding or 
post-embedding reaction?
2. Are you doing immunohistochemistry? If so, what antigen? 
Pre-embedding or post-embedding reaction?
3. What plastic are you embedding in?
4. What routine stains do you need to do?
5. What part of the brain will you be looking at? The brain is a big place.
6. Do you need to do LM and EM on the same piece of tissue/same cells?

Advice on HistoNet is free. Some of it is quite good. Checking published 
papers in refereed journals and looking at the results is always a good 
idea, espcially given the cost of experimental animals.

Geoff

Kristen Reynolds wrote:

I need advice on perfusion solutions for rat/monkey
brains.  I need to use the tissue for both light
microscopy and EM.  Any suggestions?

>Wow, I can't believe how rude of a response I got.  I
>thought this was for helpful comments.  I know how to
>perfuse for light microscopy and EM.  I just thought
>the fixative combination for wanting to do both on the
>same tissue might be out there.  I guess I won't be
>asking histonet anything anymore.
>
>__________________________________________________
>


 This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying, distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.




------------------------------

Message: 5
Date: Thu, 20 Jan 2005 11:28:47 -0800
From: Rocan <rocan <@t> mac.com>
Subject: Re: [Histonet] NIH image J surface area unit
To: "'histonet <@t> pathology.swmed.edu'" <histonet <@t> pathology.swmed.edu>,
	Subratab <subratab <@t> bdonline.com>
Message-ID: <816F081F-6B19-11D9-8D0A-000A9589219E <@t> mac.com>
Content-Type: text/plain;	charset=US-ASCII;	format=flowed

I guess there are a couple of ways to do this. I use a stage micrometer 
(you can google "stage micrometer" and find it on the pictures).  I 
take a picture of the ruler imprinted. Typically these rulers have a 
one millimeter ruler and 100 divisions of 10 micrometers each.  So, 
with the photo using the same scope and objective you go to any of 
these programs like NIH ImageJ and open the document (same size as the 
photos you are measuring). There is a command where you place the mouse 
on one end drag and click on the other end of the markings.  So, if you 
drag it over ten marking you then instruct the program that that 
distance is 100 micrometers.  Then using these settings you do your 
measurements and the measurements would then be very precise and will 
be in micrometers.  I do this with a different program but I know you 
can do it with ImageJ.
I hope this helps.
Rocio

-----
Dr.Rocio Sierra-Honigmann
Director
Engineered Wound Repair Laboratory
Cedars Sinai Research Institute
Davis 1091
310-423-1882
Honigmannr <@t> cshs.org	

On Jan 20, 2005, at 10:02 AM, Subratab wrote:

> Dear Histo experts
> My friend is measuring glomerular surface area by using Image J 
> software. He
> is in trouble to get the unit of the area calculated by the software, I
> mean, if the calculated area is to be expressed in square milimeter or
> square micrometer.
> He is capturing the figures from microscope with X100 magnification
> (oil-emersion lense) and then analyzing the glomerular surface area by 
> the
> software in a computer.
> Could anybody please help to solve this.
> Sincerely
>
> Subrata Biswas
> University of Campinas
> SP, Brazil.
>
> --
> http://www.histosearch.com/homepages/DrSubrataKumarBiswas.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 6
Date: Thu, 20 Jan 2005 12:09:42 -0800 (PST)
From: Gareth Davis <mrsgbd2001 <@t> yahoo.com>
Subject: Re: [Histonet] Certification requirements
To: Fran Lemons <flemons <@t> bhset.org>,	Histonet
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050120200942.71181.qmail <@t> web52708.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Fran,
I believe it's based on what state you work in.  I know that Florida
requires an ASCP certification as well as a Florida license.
Tennessee, where I work, does not require it.
Gareth Davis

Fran Lemons <flemons <@t> bhset.org> wrote:
Has anyone out there seen anything in writing about not being able to work
in histology labs without HT certification?
Thanks
Fran Walker


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

		
---------------------------------
Do you Yahoo!?
 Yahoo! Search presents - Jib Jab's 'Second Term'

------------------------------

Message: 7
Date: Thu, 20 Jan 2005 12:14:32 -0800 (PST)
From: Gareth Davis <mrsgbd2001 <@t> yahoo.com>
Subject: Re: [Histonet] Rudeness on histonet
To: "Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org>,	Histonet
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050120201432.90980.qmail <@t> web52710.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Here! Here! to Julie.
I have also experienced the rudeness and seen others put down and treated as
if they were stupid.  It's very discouraging.
Gareth

"Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org> wrote:
Dear Kristen and others:

I too feel that Kristen was treated rudely. I have noticed this problem from
time to time on HistoNet and I feel I have to say something. Some people
generally assume that because you ask an open question that you are
clueless. While you might get better answers by being more specific, the
better way to respond to "any suggestions" is to ask if you could provide
more details. Just assuming that you and your boss have no idea what you are
doing and have ventured into this project naively is not fair. That response
was not helpful - it was nothing but rude. Did they suggest a class or a
book that they found practically good? No! The response was condescending
and pointless. Please ask for clarification before you humiliate a person.
And please do not tell someone they deserved to be treated rudely - that is
even worse!

People write into histonet with questions because there is a wealth of
knowledge amongst the subscribers. This is valuable information and
constitutes years and years of experience. Keep in mind, all of our
experiences have been different. Just because a person might not have
experience in one area doesn't mean that they are not very knowledgeable in
another. In other words, if you are rude to a person they might not want to
share valuable information with you when you ask. Or worse, they might leave
the Histonet and deprive all of us of their knowledge! 

I too asked an open question one time about processing tissue with plastic
beads in it and received an incredibly condescending response instructing me
on how to design an experiment. I have a Ph.D. for god's sake. I have spent
15 years designing experiments. I also saw a young student publicly filleted
for cheating when her instructor encouraged her to send her inquiry out to
the Histonet. 

Kristen, please don't leave the histonet because of one (incredibly) rude
response. Take it for what it is - useless! Please hold out for helpful
people that actually have information to contribute.

Hang in there!

Julie

Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org 




Message: 2
Date: Thu, 20 Jan 2005 11:44:27 -0800
From: Geoff McAuliffe 
Subject: Re: [Histonet] perfusion suggestions follow up
To: Kristen Reynolds 
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <41F00A1B.6060101 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1

Dear Kristen:

Your original posting (see below) gave no indication that you were 
familiar with perfusion for LM or EM. You did not ask for fixative 
composition, you just asked for "any suggestions". Given the broad scope 
of your question, the advice you got WAS helpful. The fixative 
combination for wanting to do both on the same tissue IS out there, but 
you have to be more specific in your question. The correct fixative 
combinatio will depend on:
1. Are you doing histochemistry? If so, what reaction? Pre-embedding or 
post-embedding reaction?
2. Are you doing immunohistochemistry? If so, what antigen? 
Pre-embedding or post-embedding reaction?
3. What plastic are you embedding in?
4. What routine stains do you need to do?
5. What part of the brain will you be looking at? The brain is a big place.
6. Do you need to do LM and EM on the same piece of tissue/same cells?

Advice on HistoNet is free. Some of it is quite good. Checking published 
papers in refereed journals and looking at the results is always a good 
idea, espcially given the cost of experimental animals.

Geoff

Kristen Reynolds wrote:

I need advice on perfusion solutions for rat/monkey
brains. I need to use the tissue for both light
microscopy and EM. Any suggestions?

>Wow, I can't believe how rude of a response I got. I
>thought this was for helpful comments. I know how to
>perfuse for light microscopy and EM. I just thought
>the fixative combination for wanting to do both on the
>same tissue might be out there. I guess I won't be
>asking histonet anything anymore.
>
>__________________________________________________
>


This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying, distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

		
---------------------------------
Do you Yahoo!?
 Yahoo! Search presents - Jib Jab's 'Second Term'

------------------------------

Message: 8
Date: Thu, 20 Jan 2005 12:49:37 -0800 (PST)
From: Dawn Cowie <dlcowie <@t> prodigy.net>
Subject: [Histonet] certification requirements to work in histo labs
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050120204937.7153.qmail <@t> web81008.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii


Fran,

In Florida where I am, to perform tech duties you must be licensed. However,
you may employ non techs to work as lab aides. These aides may perform a
wide variety of duties that usually are done by techs. Specifically what
they cannot do is: embed, cut, stain, frozen sections etc. What they can do
is: change reagents, coverslip, start and stop an automated stainer. I hope
this helps.

Dawn Cowie, HT

Pensacola Path
 


------------------------------

Message: 9
Date: Thu, 20 Jan 2005 12:57:54 -0800
From: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>
Subject: Re: [Histonet] Rudeness on histonet
To: histonet <@t> lists.utsouthwestern.edu,	jhabecke <@t> seattlecca.org
Message-ID: <s1efaadd.026 <@t> gwsmtp.ohsu.edu>
Content-Type: text/plain; charset=us-ascii

BRAVO ladies for speaking your mind!!!! :0)
Robyn

>>> "Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org> 1/20/2005
11:27:09 AM >>>

Dear Kristen and others:

I too feel that Kristen was treated rudely. I have noticed this problem
from
time to time on HistoNet and I feel I have to say something. Some
people
generally assume that because you ask an open question that you are
clueless. While you might get better answers by being more specific,
the
better way to respond to "any suggestions" is to ask if you could
provide
more details. Just assuming that you and your boss have no idea what
you are
doing and have ventured into this project naively is not fair. That
response
was not helpful - it was nothing but rude. Did they suggest a class or
a
book that they found practically good? No! The response was
condescending
and pointless. Please ask for clarification before you humiliate a
person.
And please do not tell someone they deserved to be treated rudely -
that is
even worse!

People write into histonet with questions because there is a wealth of
knowledge amongst the subscribers. This is valuable information and
constitutes years and years of experience. Keep in mind, all of our
experiences have been different. Just because a person might not have
experience in one area doesn't mean that they are not very
knowledgeable in
another. In other words, if you are rude to a person they might not
want to
share valuable information with you when you ask. Or worse, they might
leave
the Histonet and deprive all of us of their knowledge! 

I too asked an open question one time about processing tissue with
plastic
beads in it and received an incredibly condescending response
instructing me
on how to design an experiment. I have a Ph.D. for god's sake. I have
spent
15 years designing experiments. I also saw a young student publicly
filleted
for cheating when her instructor encouraged her to send her inquiry out
to
the Histonet. 

Kristen, please don't leave the histonet because of one (incredibly)
rude
response. Take it for what it is - useless! Please hold out for
helpful
people that actually have information to contribute.

Hang in there!

Julie

Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org <mailto:jhabecke <@t> fhcrc.org>




Message: 2
Date: Thu, 20 Jan 2005 11:44:27 -0800
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] perfusion suggestions follow up
To: Kristen Reynolds <kristenhinkle <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <41F00A1B.6060101 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1

Dear Kristen:

    Your original posting (see below) gave no indication that you were

familiar with perfusion for LM or EM. You did not ask for fixative 
composition, you just asked for "any suggestions". Given the broad
scope 
of your question, the advice you got WAS helpful. The fixative 
combination for wanting to do both on the same tissue IS out there, but

you have to be more specific in your question. The correct fixative 
combinatio will depend on:
1. Are you doing histochemistry? If so, what reaction? Pre-embedding or

post-embedding reaction?
2. Are you doing immunohistochemistry? If so, what antigen? 
Pre-embedding or post-embedding reaction?
3. What plastic are you embedding in?
4. What routine stains do you need to do?
5. What part of the brain will you be looking at? The brain is a big
place.
6. Do you need to do LM and EM on the same piece of tissue/same cells?

Advice on HistoNet is free. Some of it is quite good. Checking
published 
papers in refereed journals and looking at the results is always a good

idea, espcially given the cost of experimental animals.

Geoff

Kristen Reynolds wrote:

I need advice on perfusion solutions for rat/monkey
brains.  I need to use the tissue for both light
microscopy and EM.  Any suggestions?

>Wow, I can't believe how rude of a response I got.  I
>thought this was for helpful comments.  I know how to
>perfuse for light microscopy and EM.  I just thought
>the fixative combination for wanting to do both on the
>same tissue might be out there.  I guess I won't be
>asking histonet anything anymore.
>
>__________________________________________________
>


This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying,
distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 10
Date: Thu, 20 Jan 2005 12:58:14 -0800 (PST)
From: Georgia Stewart <stewart_georgia <@t> sbcglobal.net>
Subject: [Histonet] HIPPA rules and fax transmittals going to wrong
	person
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050120205814.70166.qmail <@t> web81402.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hello and Good Afternoon Everyone
 
In this era of HIPPA rules and regulations in the United States, I have not
seen any comment on HIPPA as it relates to fax transmittal's of reports that
might go to the wrong person/facility.  If a report goes to the the wrong
person/facility, isn't this is a breach of patient confidentiality covered
by HIPPA?  How are you handling faxs that go, or might go, to the wrong
person/facility?  
 
Appreciate your comments.
 
Georgia Stewart, BS, HTL



Georgia


------------------------------

Message: 11
Date: Thu, 20 Jan 2005 15:00:39 -0600
From: "Rittman, Barry" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] perfusion suggestions
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<566FB0B522443D43AF02D2ADBE35A6F0012C92C3 <@t> UTHEVS3.mail.uthouston.edu>
Content-Type: text/plain;	charset="us-ascii"

Kristen
I am not trying to justify any response that you received but I think
that I understand why the response was phrased in that particular
manner. 
Many of the questions that arrive at Histonet are very specific, and
generally get a lot of responses. 
The broader questions are often difficult to answer (and in general I
personally do not respond to these) and often receive few responses.  
It is usually not possible to determine the level of knowledge of the
individual who is asking the question and there may be a wide variety of
answers. 
Here on Histonet we are often between a rock and a hard place. I believe
that it is a natural tendency nowadays to pose the question in the
shortest way possible on the assumption that nobody wishes to spend
their time to read a long discourse. This unfortunately often results in
a communication that is open to a wide variety of interpretations. One
interpretation is that the individual posing the question wants to be
informed about all aspects of a particular technique from anesthetizing
an animal to interpreting the final result, rather than carrying out the
initial groundwork themselves. There is usually no way to judge if this
is true from the original communications.
This is the era of rapid fire information but unfortunately the
recipients do not always have the same background or experiences to view
that information in the same light. 
In many cases I feel that in response to complex questions, individuals
with the appropriate expertise should provide their telephone number so
that a dialogue can be set up. While Histonet is very useful, a one on
one dialogue generally will provide the most relevant information.
I hope that you will continue to ask questions on Histonet.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristen
Reynolds
Sent: Thursday, January 20, 2005 9:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] perfusion suggestions

Wow, I can't believe how rude of a response I got.  I
thought this was for helpful comments.  I know how to
perfuse for light microscopy and EM.  I just thought
the fixative combination for wanting to do both on the
same tissue might be out there.  I guess I won't be
asking histonet anything anymore.

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------------------------------

Message: 12
Date: Thu, 20 Jan 2005 13:24:08 -0800
From: Victor Tobias <victor <@t> pathology.washington.edu>
Subject: Re: [Histonet] HIPPA rules and fax transmittals going to
	wrong person
To: Georgia Stewart <stewart_georgia <@t> sbcglobal.net>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <41F02178.1080606 <@t> pathology.washington.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed

We had an incident where a patient report went to a Barnes and Noble 
bookstore. At the time of the incident many users had access to our 
database for inputting new referring physicians. It is very easy to make 
a typo and the fax is going who knows where. The bookstore contacted us 
regarding the incident. We confirmed that we had their fax number in our 
database. An incident report was filed with risk management. Since the 
incident, we have restricted access to our database. Our facility 
recommendation is to verify fax numbers quarterly. With our staffing 
levels this is not feasible. We do send out annual verifications, that 
whoever signs the form is stating the fax number is correct and the 
machine is in a secure location. We will make several attempts to get 
the verification back. If there is no response then we will stop faxing 
to them and send a print copy through the mail. We have custom reports 
created to query the database when a physician is listed on a current 
case and their fax verification is more than a year old. Our facility 
policy states that a reasonable effort will be made to verify a fax 
number. What happens when a doctors office calls and asks for a copy of 
a patient report? We document in the database who we sent a copy of the 
report to.

Victor

Georgia Stewart wrote:

>Hello and Good Afternoon Everyone
> 
>In this era of HIPPA rules and regulations in the United States, I have not
seen any comment on HIPPA as it relates to fax transmittal's of reports that
might go to the wrong person/facility.  If a report goes to the the wrong
person/facility, isn't this is a breach of patient confidentiality covered
by HIPPA?  How are you handling faxs that go, or might go, to the wrong
person/facility?  
> 
>Appreciate your comments.
> 
>Georgia Stewart, BS, HTL
>
>
>
>Georgia
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>

-- 
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
=================================================
Privileged, confidential or patient identifiable information may be
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of the intended recipients. If you are not the intended recipient, or 
if the message has been addressed to you in error, do not read, 
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transmission. Instead, please notify the sender by reply e-mail, and 
then destroy all copies of the message and any attachments.




------------------------------

Message: 13
Date: Thu, 20 Jan 2005 22:04:33 GMT
From: "mprice26 <@t> juno.com" <mprice26 <@t> juno.com>
Subject: [Histonet] RE: Rudeness on Histonet
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050120.140511.23783.21286 <@t> webmail26.nyc.untd.com>
Content-Type: text/plain


I have been treated rudely also. I have e-mailed the histonet for a vendor's
# or for other info and I will have people rerspond back with try google
search.

I know how to search for something via google or yellowpages.com but prefer
to use the histonet because of the vast number of vendors that subscribe to
the histonet. So not only do I receive the # I am asking for I also receive
info from other vendors that I would not receive if I look it up on google.

I wish those that think I am an idiot for not using google would just not
respond to my request. If it bothers them so much that I am requesting info
via the Histonet. It takes much less time to hit their delete button than it
does for them to e-mail me a rude, condescending message.

I would like to add that for the most part I receive prompt useful replys,
it is just a few people that reply with rude answers.

Marsha Price



------------------------------

Message: 14
Date: Thu, 20 Jan 2005 14:13:17 -0800 (PST)
From: pam plumlee <paw555 <@t> yahoo.com>
Subject: [Histonet] GLP lab staining question
To: HistoNet Server <histonet <@t> pathology.swmed.edu>
Message-ID: <20050120221317.48866.qmail <@t> web11605.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hello fellow histonetters:  I'm setting up a special
stain control/slide bank for our research pharm lab. 
I'm curious how special stain controls are validated. 
Is it as simple as screening the stained slides/blocks
as they are cut and determining if they are positive
or not, along with the proper documentation?  Anyone
care to share info?  Thanks, PP


		
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Take Yahoo! Mail with you! Get it on your mobile phone. 
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------------------------------

Message: 15
Date: Thu, 20 Jan 2005 15:14:25 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] certification requirements to work in histo
	labs
To: Dawn Cowie <dlcowie <@t> prodigy.net>,
	Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20050120150521.01b1fbc0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

I am curious or rather need clarification here.  Licensed means licensed by 
the state but HT(SCP) is a certification granted by a certification agency, 
ASCP/CAP.  I don't think ASCP/CAP is governed by any state's' licensing 
regulations.

Many moons ago, in New York, one did not have to be certified as an HT to 
be licensed by the state (one had to take a test for NY licensure), and 
there were NY licensed technicians without HT(ASCP) certification 
performing tech duties and, in fact, was the supervisor of our 
histopathology lab.



At 01:49 PM 1/20/2005, you wrote:

>Fran,
>
>In Florida where I am, to perform tech duties you must be licensed. 
>However, you may employ non techs to work as lab aides. These aides may 
>perform a wide variety of duties that usually are done by techs. 
>Specifically what they cannot do is: embed, cut, stain, frozen sections 
>etc. What they can do is: change reagents, coverslip, start and stop an 
>automated stainer. I hope this helps.
>
>Dawn Cowie, HT
>
>Pensacola Path
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)





------------------------------

Message: 16
Date: Thu, 20 Jan 2005 14:36:24 -0800
From: Bryan Llewellyn <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] GLP lab staining question
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001601c4ff40$7923b6b0$80004246 <@t> yourlk4rlmsu>
Content-Type: text/plain; reply-type=original; charset=iso-8859-1;
	format=flowed

The way I was taught was to cut a series of sections, 50 or so, then stain 
the first and the last to make sure they were positive  If they both are, it

is presumed that all the others are also.  If not, stain intermediate 
sections until one is positive, then use from the first one to that.

Bryan Llewellyn

----- Original Message ----- 
From: "pam plumlee" <paw555 <@t> yahoo.com>
To: "HistoNet Server" <histonet <@t> pathology.swmed.edu>
Sent: Thursday, January 20, 2005 2:13 PM
Subject: [Histonet] GLP lab staining question


> Hello fellow histonetters:  I'm setting up a special
> stain control/slide bank for our research pharm lab.
> I'm curious how special stain controls are validated.
> Is it as simple as screening the stained slides/blocks
> as they are cut and determining if they are positive
> or not, along with the proper documentation?  Anyone
> care to share info?  Thanks, PP
>
>
>
> __________________________________
> Do you Yahoo!?
> Take Yahoo! Mail with you! Get it on your mobile phone.
> http://mobile.yahoo.com/maildemo
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 17
Date: Thu, 20 Jan 2005 16:45:10 -0600
From: "Linda  Jones" <ljones <@t> pathology.umsmed.edu>
Subject: [Histonet] Special stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s1efe01d.082 <@t> GWIA1.umsmed.edu>
Content-Type: text/plain; charset=US-ASCII

How do you charge for certain stains? Should charge due to the length of
time it take to do the stain or the price of the chemicals? 
thanks


Linda Harper-Jones BS.,HT/ HTL(ASCP)
University of Mississippi Medical Center
Department of Pathology
Chief Histotechnologist Supervisor
(601) 984-1576
(601) 984-4968 Fax
ljones <@t> pathology.umsmed.edu



------------------------------

Message: 18
Date: Thu, 20 Jan 2005 15:49:45 -0800
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Special stain
To: "Linda Jones" <ljones <@t> pathology.umsmed.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	
<OF0D388D8F.CA46DB2D-ON88256F8F.0082BFE2-88256F8F.0083B446 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

Special stains are based on the type of stain.   For billing purposes 
there are two types:  microorganism or not.  88312 and 88313.  Cost or 
length of time is not a factor when billing. 





"Linda  Jones" <ljones <@t> pathology.umsmed.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/20/2005 02:45 PM

To
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Special stain






How do you charge for certain stains? Should charge due to the length of
time it take to do the stain or the price of the chemicals? 
thanks


Linda Harper-Jones BS.,HT/ HTL(ASCP)
University of Mississippi Medical Center
Department of Pathology
Chief Histotechnologist Supervisor
(601) 984-1576
(601) 984-4968 Fax
ljones <@t> pathology.umsmed.edu

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 19
Date: Fri, 21 Jan 2005 04:04:26 +0000
From: Kim O'Sullivan <Kim.Osullivan <@t> med.monash.edu.au>
Subject: [Histonet] Bouins fixation problem
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <130.194.114.210.1106279796.85555 <@t> my.monash.edu.au>
Content-Type: text/plain

Hi everybody,

We have used bouin's fixative for fixing mouse kidneys for years with no
problem, and have recently been experiencing problems cutting them once they
have been processed and wax embedded. The  kidney tissue has holes in it in
the medulla region, is very difficult to cut(tissue brittle) and it appears
almost like the wax has not infiltrated the kidneys properly. Additonally
once the sections have been mounted on glass slides they appear fine but
when we go to PAS stain them half of them come off the slides in solution
(this has never happened before in the last 3 years)I have checked the
processing schedule and that is fine (as is all the solutions) I have
checked the temperature of the wax (which is fine). Does anyone out there
know what our problem may be?? Additionally is there any way that any of the
solutions used to make bouin's (picric acid, formalin and glacial acetic
acid)can go off? And how would you check for this.

Would appreciate any help!!

Kim O'Sullivan



------------------------------

Message: 20
Date: Fri, 21 Jan 2005 05:24:15 -0800 (PST)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] c-fos antibody in mouse tissue
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050121132416.20426.qmail <@t> web52502.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hello All,
 
I was wondering if anyone knew what tissue would make the best positive
control for this antibody in mouse tissue.
 


Kim Merriam
Novartis
Cambridge, MA
__________________________________________________
Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
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------------------------------

Message: 21
Date: Fri, 21 Jan 2005 09:23:30 -0500
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] Coverslipping tape
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s1f0ca25.034 <@t> GHSGWIANW2.GEISINGER.EDU>
Content-Type: text/plain; charset=US-ASCII

What is the concensus of opinion on Mercedes Medicals coverslipping
tape?
I got a sample to try and the girls I work with said they had it before,
(I don't know how long ago) and in a couple days it started lifting. 

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916


IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.

------------------------------

Message: 22
Date: Fri, 21 Jan 2005 09:25:41 EST
From: DPALLP <@t> aol.com
Subject: [Histonet] saturated fingertips
To: histonet <@t> pathology.swmed.edu
Message-ID: <191.372a8459.2f226ae5 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

Does anyone have any suggestions to alleviate water saturated fingertips  
from handling blocks from ice trays?   Wearing gloves is too  cumbersome and
the 
different barrier lotions that we have tried do not seem to  have an effect.

 
Susie


------------------------------

Message: 23
Date: Fri, 21 Jan 2005 09:39:29 -0800
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Bouins fixation problem
To: Kim O'Sullivan <Kim.Osullivan <@t> med.monash.edu.au>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <41F13E51.1010507 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1

Hi Kim:

    Hmm. Certainly sounds like an infiltration problem. My first though 
is that you are not getting all of the alcohol out of the medulla so 
xylene (or substitute) is not getting in so the wax is not getting in.  
When you say that you have checked all of the processing solutions and 
they are fine, how did you check them? What is the criteria for deciding 
that a solution is good? I would dump ALL of  the processing solutions 
and ALL of the wax and start fresh. Run some kidneys, cut them, and see 
if that solves the problem. There is a small chance that the 
manufacturer of your wax has changed the formula but with modern waxes 
and additives such a change seems unlikely to cause problems.
    As for reagents going bad, picric acid last forever, formaldehyde is 
fine as long as there is no ppt. on the bottom of the bottle, I don't 
think glacial acetic acid goes bad. Even if the fixation was incomplete 
(you did not say how long you fix the kidneys, if you perfuse fixative, 
or if you slice them open) the alcohols should finish the fixation.
    Good luck!

Geoff

Kim O'Sullivan wrote:

>Hi everybody,
>
>We have used bouin's fixative for fixing mouse kidneys for years with no
problem, and have recently been experiencing problems cutting them once they
have been processed and wax embedded. The  kidney tissue has holes in it in
the medulla region, is very difficult to cut(tissue brittle) and it appears
almost like the wax has not infiltrated the kidneys properly. Additonally
once the sections have been mounted on glass slides they appear fine but
when we go to PAS stain them half of them come off the slides in solution
(this has never happened before in the last 3 years)I have checked the
processing schedule and that is fine (as is all the solutions) I have
checked the temperature of the wax (which is fine). Does anyone out there
know what our problem may be?? Additionally is there any way that any of the
solutions used to make bouin's (picric acid, formalin and glacial acetic
acid)can go off? And how would you check for this.
>
>Would appreciate any help!!
>
>Kim O'Sullivan
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>  
>

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
**********************************************






------------------------------

Message: 24
Date: Fri, 21 Jan 2005 10:00:37 -0500
From: "Rice, Michael" <Michael.Rice <@t> holy-cross.com>
Subject: RE: [Histonet] Coverslipping tape
To: "Angela Bitting" <akbitting <@t> geisinger.edu>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<3BC92F29BE821745AB15E04C98EE028D693742 <@t> HCH2KMAIL.holy-cross.com>
Content-Type: text/plain

I have been using it for several months now and have seen no evidence of
lifting, not to say that it wont happen in the future. A friend of mine has
beeen using it for several years now with no problems
Mike Rice
Holy Cross hospital
ft lauderdale

-----Original Message-----
From: Angela Bitting [mailto:akbitting <@t> geisinger.edu]
Sent: Friday, January 21, 2005 9:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Coverslipping tape


What is the concensus of opinion on Mercedes Medicals coverslipping
tape?
I got a sample to try and the girls I work with said they had it before,
(I don't know how long ago) and in a couple days it started lifting. 

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916


IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


----------------------
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Attorney-Client Privileged. This e-mail message, including any attachments,
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------------------------------

Message: 25
Date: Fri, 21 Jan 2005 09:01:05 -0600
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: [Histonet] RE: Rudeness on Histonet
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <D2401DE71F59D71184BC00D0B7479B91A58D93 <@t> MILW_MAIL1>
Content-Type: text/plain;	charset="iso-8859-1"

Rude people on a listserver like this are unavoidable.  Unfortunately, there
is no "pre-membership rudeness testing".  If you are tired of being chewed
on like a bloody wildabeast in the midst of a den of lions, do what I was
forced to do and avoid lengthy general postings as much as possible.  Submit
a pithy comment/question and pursue only those who seem to genuinely want to
help and delete those who are jerks.  Trying to rail against rudeness can
become a full time job which doesn't pay anything.  

Histonet is too valuable a tool to just get rid of, though, at times, it is
tempting.

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI



------------------------------

Message: 26
Date: Fri, 21 Jan 2005 10:10:31 EST
From: KarBieber <@t> aol.com
Subject: [Histonet] Use of Sta-On for Immuno stains
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <194.36bbc139.2f227567 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

I was just wondering how other labs are handling this situation:  For 
prostate and breast core biopsies we cut extra slides for potential immuno
staining, 
between the levels.  The problem is the use of adhesive in the water bath.  
Since we are cutting H&E's, there is the possibility of fall off during
staining 
if we DON'T use sta-on.  But using it increases the possibility of fall off 
during immunos staining.  And if we don't use the sta-on, we run the risk of

fall off on ALL the cases the techs are cutting that day.  

I know there are lots of labs out there performing immunos, so I'd like to 
know how you've resolved this.
Thanks,
Karen


------------------------------

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End of Histonet Digest, Vol 14, Issue 26
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