[Histonet] formic acid / Nasd on bone marrow

[JOHN PHILLIPS]

Esterase activity is lost after acid decalcification. Investigations for naphthol as-d chloroacetate esterase for granulocytes and alpha-naphthyl acetate esterase for monocytes will not work. Both investigations work fine after ADTA decal.

John,
Wrexham, UK.

-----Original Message-----
From: Gudrun Lang [mailto:gu.lang <@t> gmx.at]
Sent: 19 January 2005 12:24
To: Histonetliste (Histonetliste)
Subject: [Histonet] formic acid / Nasd on bone marrow


To those, who use formic acid to decal bone marrow trephines.

Have you experience with performing Naphtol-AS-D-chloroacetat-esterase on
your slides?

 

Until now we decal with buffered EDTA an our NASD works well. Immunos show
some difficulties. So we start to test formic acid.

I would like to know, if the esterase is influenced by the acid.

 

Thank you

Gudrun Lang

Akh Linz, Austria



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