[Histonet] Myelin basic Protein in mouse

[Marceau, Gabriel (IAF)(LAVAL)]

For those it may concern the product in question was sold as an ascite until 2003 (found an old catalogue) and now it is sold only as a culture supernatant. It is the same epitope, same clone, etc. They probably cannot make ascite anymore for the same reasons we can no longuer do that here at the research center. The animal care committee is restricting ascite production.

Sadly for the use I need the antibody for it will cost too much to use that product if I cannot dilute it. I want to label myelin on spinal cord sections (cryostat sections of 4%para fixed tissu) but I have "alot" of slides to label... 

Gabriel

------------------------------------------------------------
Gabriel Marceau
INRS-Institut Armand-Frappier
531, boul. des Prairies
Laval (Québec) H7V 1B7

-----Original Message-----
Sent: Wed 19/01/05 10:08
To: Marceau, Gabriel (IAF)(LAVAL); histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Myelin basic Protein in mouse
 
Hi Gabriel,
I see your dilema.  The 2004 Serotec catalog lists MCA408S as a supernatant.
Can't find my old catalog and I haven't ordered for a while but my vial is
250 ul of MCA408 (no S) and is batch 050599.  It is from Aug 2001 and still
working nicely at 1:2000 or so.  Maybe a call to company can trace why the
#'s are different.  Possibly clone died off or drifted or they are trying to
re-establish it in vivo???

Ray
Raymond Koelling
Research Scientist-Pathology
Amgen Corp.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Marceau,
Gabriel (IAF)(LAVAL)
Sent: Wednesday, January 19, 2005 6:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Myelin basic Protein in mouse


Ray,

Serotec has a Rat anti-MBP under the catalogue number MCA408S but it is a
culture supernatant, is this what you are using at the dilution of 1:2000?
Usually supernatant are used pure or nearly-pure this is why I ask. If not
can you still order number MCA408 even if no longuer in the catalogue?

Thanks,

Gabriel

------------------------------------------------------------
Gabriel Marceau
INRS-Institut Armand-Frappier
531, boul. des Prairies
Laval (Québec) H7V 1B7
Tél.: (450) 687-5010 poste 4282


From:	"Koelling, Ray"

Cynthia,
for a couple of years we've run rat-antiMBP from Serotec (MCA408) on mouse
brain fixed 24 hours in NBF.  At 1:1000-1:2000 overnight at 4 degrees with
no retrieval what-so-ever. Then biotinylated rabbit anti rat and so forth.
Ray
Raymond Koelling
Research Scientist, Pathology
Amgen Corp.
Seattle, WA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Favara,
Cynthia (NIH/NIAID)
Sent: Friday, January 07, 2005 11:21 AM
To: Histonet
Subject: [Histonet] Myelin basic Protein in mouse


I have run a quick search in the archives and found nothing. Anyone with
experience with Myelin Basic Protein  immunohistochemistry in FFPE murine
tissue?

Thanks,
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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