[Histonet] Frozen section of plastic stent

[Alan Bright]

Dear Cathy & others with this problem,

I do not know the answer to your problem with sectioning plastic
(silicone) stents, but I have experience of sectioning a wide range of
materials including plastics. If you could send me a sample I would be
very happy to section the stent and give you a full report with no costs
to you. I am hoping that all that is required is determining the correct
temperature and maybe using a tungsten carbide tipped knife.


Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Cathy M. Mathis [mailto:cmmathis <@t> wfubmc.edu] 
Sent: 03 January 2005 21:51
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Frozen section of plastic stent


Happy New Year Histonetters!!
Any suggestions for cutting plastic (silicone) stent embedded in OCT on
the cryostat.  I'm cutting at 10 microns, -28 C and about every third or
fourth section is good.  I am moving the blade every 20-30 sections.
Most of the sections are badly folded or fractured.  The stent will
eventually be part of tissue, farther into the block. Thanks in advance
for the help. Cathy
 
Cathy M. Mathis, H.T. (ASCP)
Institute of Regenerative Medicine
Wake Forest University Health Sciences
Tissue Engineering Laboratory
NRC Bldg.  Rm. 126
Medical Center Blvd.
Winston-Salem, NC   27157
ph:  336-713-7285
email: cmmathis <@t> wfubmc.edu
 
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