[Histonet] RE: CD68 in monkey frozen tissue

[C.M. van der Loos]

A HAPPY AND HEALTHY NEW YEAR TO EVERYONE TOO!

CD68, clone PG-M1 does stain something on frozen sections. However, if 
this staining result reflects the full spectrum of macrophages is a bit 
doubtful. For frozens you better use CD68 clone EBM11.

Best wishes,
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands

phone:  +31 20 5665631
fax:    +31 20 6960389
e-mail: c.m.vanderloos <@t> amc.uva.nl

----- Original Message ----- 
>From  antje.marcantonio <@t> pharma.novartis.com 
Date  Mon, 3 Jan 2005 11:37:43 +0100 
To  histonet <@t> lists.utsouthwestern.edu 
Subject  [Histonet] CD68 in monkey frozen tissue 


HAPPY NEW YEAR TO EVERYONE !


Hello 

I use the anti human  CD68, KP1 from Dako on FFPE monkey tissue with 
good 
results. The same antibody is not recommended for frozens. 
I tried it anyway and it is indeed difficult to get an enough strong 
signal without background. 
What about the other clone PG-M1 ? Is this working on frozen sections ?

Any input is very much welcome !

Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland
Tel. +41 61 324 6730
antje.marcantonio <@t> pharma.novartis.com