[Histonet] Frozen section of plastic stent

[Patsy Ruegg]

Cathy I would use the tape transfer system from Instrumedics to hold
everything together but you will probably need to use steel or tungsten
carbide knives.  
Patsy Ruegg 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cathy M.
Mathis
Sent: Monday, January 03, 2005 2:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Frozen section of plastic stent

Happy New Year Histonetters!!
Any suggestions for cutting plastic (silicone) stent embedded in OCT on the
cryostat.  I'm cutting at 10 microns, -28 C and about every third or fourth
section is good.  I am moving the blade every 20-30 sections.
Most of the sections are badly folded or fractured.  The stent will
eventually be part of tissue, farther into the block.
Thanks in advance for the help.
Cathy
 
Cathy M. Mathis, H.T. (ASCP)
Institute of Regenerative Medicine
Wake Forest University Health Sciences
Tissue Engineering Laboratory
NRC Bldg.  Rm. 126
Medical Center Blvd.
Winston-Salem, NC   27157
ph:  336-713-7285
email: cmmathis <@t> wfubmc.edu
 
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