[Histonet] Frozen section of plastic stent

[Cathy M. Mathis]

Happy New Year Histonetters!!
Any suggestions for cutting plastic (silicone) stent embedded in OCT on
the cryostat.  I'm cutting at 10 microns, -28 C and about every third or
fourth section is good.  I am moving the blade every 20-30 sections.
Most of the sections are badly folded or fractured.  The stent will
eventually be part of tissue, farther into the block.
Thanks in advance for the help.
Cathy
 
Cathy M. Mathis, H.T. (ASCP)
Institute of Regenerative Medicine
Wake Forest University Health Sciences
Tissue Engineering Laboratory
NRC Bldg.  Rm. 126
Medical Center Blvd.
Winston-Salem, NC   27157
ph:  336-713-7285
email: cmmathis <@t> wfubmc.edu