(Fwd) Re: [Histonet] EDTA pH 8.0 recipe

[Gayle Callis]

I would like to point that he did NOT specify with EDTA he was using,
presumption is EDTA or EDTA disodium.  Unfortunately, this is a pet peeve
of mine, one should always say WHICH EDTA is being used, or at least give a
formula weight. 
>
>Stock Solution:
>	1.86 gm. EDTA (same as used for decalcification)
>	5.0   liters DI water
>
>To the above solution, add 2 to 2.5 ml. 2N NaOH.
>Adjust solution to pH 8.0 with 1N NaOH or 1N HCl solution.
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
>From:           	"Barnhart, Tammy" <Tbarnhart <@t> primecare.org>
>To:             	"Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
>Date sent:      	Wed, 24 Mar 2004 07:57:44 -0600
>Subject:        	[Histonet] EDTA pH 8.0 recipe
>
>> Does anyone have a recipe to make EDTA buffer at a pH of 8.0.  I would like
>> to make our own in an effort to save some dollars.  I have checked the
>> archives and only have come up with one rather difficult procedure. Thanks
>> in advance....
>> 
>> Tammy Barnhart, BS, HTL(ASCP)
>> Anatomic Pathology Supervisor
>> St. Alexius Medical Center
>> Bismarck, ND
>> tbarnhart <@t> primecare.org
>> 
>> 
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)