(Fwd) Re: [Histonet] EDTA pH 8.0 recipe

[Greg Dobbin]

Hi Tammy,
I maintain my own "personal" Histonet Archives, and I found the 
following post from 1999. Good luck.
Greg

Date sent:      	Mon, 27 Dec 1999 11:15:29 -0600
From:           	"Lott, Robert" <Robert.Lott <@t> bhsala.com>
Subject:        	EDTA-NaOH, pH 8.0 retrieval fluid
Forwarded to:   	DOBBIN <@t> acad1.cs.upei.ca
To:             	"'histonet <@t> pathology.swmed.edu'" 
<histonet <@t> pathology.swmed.edu>


Stock Solution:
	1.86 gm. EDTA (same as used for decalcification)
	5.0   liters DI water

To the above solution, add 2 to 2.5 ml. 2N NaOH.
Adjust solution to pH 8.0 with 1N NaOH or 1N HCl solution.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From:           	"Barnhart, Tammy" <Tbarnhart <@t> primecare.org>
To:             	"Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Date sent:      	Wed, 24 Mar 2004 07:57:44 -0600
Subject:        	[Histonet] EDTA pH 8.0 recipe

> Does anyone have a recipe to make EDTA buffer at a pH of 8.0.  I would like
> to make our own in an effort to save some dollars.  I have checked the
> archives and only have come up with one rather difficult procedure. Thanks
> in advance....
> 
> Tammy Barnhart, BS, HTL(ASCP)
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> Bismarck, ND
> tbarnhart <@t> primecare.org
> 
> 
> 
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