[Histonet] Best Way to obtain specific area of mouse foetal
and neonatal Brain for EM and light microscopy
Jo Dee Fish
jfish <@t> gladstone.ucsf.edu
Tue Apr 27 11:22:03 CDT 2004
Dear Rowani,
Here is what I do to select specific areas of the brain for EM.
First, cut 100um vibratome sections after the initial glutaraldehyde
fixation. Then process these sections through dehydration and
infiltration with Eponate 12. Embed them between Aclar sheets (I buy
mine from Ted Pella) with just a small drop of resin for each
section. I have embedded at least 50 sections between two 8x6"
sheets. After they have polymerized you can look at the sections
with an ordinary upright microscope. You can then select specific
areas and mount them onto empty or "blank" resin blocks (that you
have made ahead of time). Just remove the area you have chosen with
a razor blade or scalpel. You might practice first before going for
the important and rare specimen! I use regular gel type Super Glue
to glue the areas to the blocks. After they have dried you can trim
and section just like any other EM block.
The nice benefit of this method is that the embedded sheets can be
stored in an ordinary binder until you need them again.
This works great for me, hope it works great for you.
Take care and good luck,
Jo Dee
At 9:08 AM +0930 4/27/04, Rowani wrote:
>Yes, its electron microscopy but what I meant is to cut specific area of the
>brain (ie. hippocampus, motor cortex etc) which could then be used for EM
>and various other purposes.
>
>Thank You
>Rowani
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
**********************************************************************
**********
Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease
Telephone: (415) 695-3720
Fax: (415) 285-5632
E-mail: jfish <@t> gladstone.ucsf.edu
Mailing address:
Gladstone Institutes
P.O. Box 419100
San Francisco, CA 94141-9100
More information about the Histonet
mailing list