[Histonet] Coverslipper Agony

[Joanne Mauger]

Pat,
We had crazy problems with our Hacker too, until I realized we were using a toluene based mounting medium(which was recommended by the salesperson!) but we were mounting from xylene. The toluene dries much quicker, and not only did we get bubbles, after a while the coverslips were popping off! Switch to a xylene based mountant like Protocol from Fisher and hopefully your problem will be solved.
Good luck,
Jo

>>> "Patterson, Patricia" <Patricia.Patterson <@t> uhhs.com> 04/20/04 02:08PM >>>
Hi All!

I'd like some suggestions from you - we have a Hacker Coverslipper - using
glass coverslips - and we get air bubbles.  Not all the time, but enough to
drive us crazy.  We try to keep the dispensing needle clean and the amount
of xylene in the rack holder consistent.

What is everyone else doing??

Thanks
Pat Patterson
University Hospitals of Cleveland.


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